Serum folate and cytokines in heterozygous beta-thalassemia

Autor(es)

Paniz, Clovis ^{[1, 2]} ; Lucena, Maylla Rodrigues ^[3] ; Bertinato, Juliano Felix ^[1] ; dos Santos, Magnun Nueldo Nunes ^[4] ; Gomes, Guilherme Wataru ^[1] ; Figueiredo, Maria Stella ^[3] ; Sonati, Maria de Fatima ^[4] ; Blaia-D Avila, Vera Lucia Nascimento ^[5] ; Green, Ralph ^[6] ; Guerra-Shinohara, Elvira Maria ^{[1, 3, 7]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicol, Sao Paulo - Brazil

^[2] Univ Fed Santa Maria, Dept Anal Clin & Toxicol, Santa Maria, RS - Brazil

^[3] Univ Fed Sao Paulo, Disciplina Hematol & Hemoterapia, Sao Paulo - Brazil

^[4] Univ Estadual Campinas, Fac Ciencias Med, Dept Patol Clin, Sao Paulo - Brazil

^[5] Pontificia Univ Catolica Sao Paulo, Fac Med & Ciencias Saude, Div Hematol, Sorocaba - Brazil

^[6] UC Davis Sch Med, Dept Pathol & Lab Med, Sacramento, CA - USA

^[7] Univ Fed Mato Grosso do Sul, Fac & Ciencias Farmaceut Alimentos & Nutr, Campo Grande, MS - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY; v. 42, n. 6, p. 718-726, DEC 2020.
• Citações Web of Science: 0

Resumo

Introduction Folate deficiency is commonly reported in beta-thalassemia. Individuals heterozygous for beta-thalassemia may have higher folate requirements than normal individuals. Objectives: To document the concentration of serum total folate and its forms in beta-thalassemia heterozygote users (beta-TmU) and nonusers (beta-TmN) of 5 mg folic acid/d; to determine whether folic acid (FA) consumption from fortified foods allows beta-Tm patients, who do not take FA supplements, to meet their dietary folate requirements; and to investigate the association between higher serum unmetabolized folic acid (UMFA) and inflammatory cytokine concentrations. Methods Serum total folate and forms were measured in 42 beta-Tm (13 beta-TmU and 29 beta-TmN) and 84 healthy controls. The mononuclear leucocyte mRNA expression of relevant genes and their products and hematological profiles were determined. Results beta-TmU had higher serum total folate, 5-methyltetrahydrofolate, UMFA, and tetrahydrofolate (THF) compared with beta-TmN. The beta-TmN had lower serum total folate and THF than controls. Plasma total homocysteine (tHcy) was lower in beta-TmU compared with both beta-TmN and controls, while beta-TmN had higher tHcy than controls. beta-TmU had higher IL-8 than their controls while beta-TmN had higher IL-6 and IL-8 than their controls. beta-TmU have higher levels of serum total folate, 5- methyltetrahydrofolate, UMFA, and THF than controls. There was no association between UMFA concentrations and cytokine levels. Conclusions Mandatory flour fortification with FA in Brazil may be insufficient for beta-TmN, since they have higher tHcy and lower serum total folate than controls. Furthermore, beta-TmN have higher IL-6 levels than beta-TmU. UMFA was not associated with inflammatory cytokine levels. (AU)

• Processo FAPESP: 12/12912-1 - Efeitos do ácido fólico não metabolizado na metilação global do DNA, na expressão de RNAm e na citotoxicidade das células NK em indivíduos expostos a fortificação compulsória com ácido fólico e/ou em uso terapêutico desta vitamina
• Beneficiário: Elvira Maria Guerra Shinohara
• Modalidade de apoio: Auxílio à Pesquisa - Regular