| Texto completo | |
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Sant'Anna, Morena Brazil
[1]
;
Giardini, Aline C.
[1]
;
Ribeiro, Marcio A. C.
[2]
;
Lopes, Flavia S. R.
[1]
;
Teixeira, Nathalia B.
[1]
;
Kimura, Louise F.
[1]
;
Bufalo, Michelle C.
[1]
;
Ribeiro, Orlando G.
[3]
;
Borrego, Andrea
[3]
;
Cabrera, Wafa H. K.
[3]
;
Ferreira, Julio C. B.
[2, 4]
;
Zambelli, Vanessa O.
[5, 1]
;
Sant'Anna, Osvaldo A.
[6]
;
Picolo, Gisele
[1]
Número total de Autores: 14
|
| Afiliação do(s) autor(es): | [1] Butantan Inst, Lab Pain & Signaling, Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Biomed Sci, Sao Paulo - Brazil
[3] Butantan Inst, Immunogenet Lab, Sao Paulo - Brazil
[4] Stanford Univ, Sch Med, Dept Chem & Syst Biol, Stanford, CA - USA
[5] Stanford Univ, Sch Med, Dept Anesthesiol Perioperat & Pain Med, Stanford, CA 94305 - USA
[6] Butantan Inst, Lab Immunochem, Sao Paulo - Brazil
Número total de Afiliações: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | FRONTIERS IN IMMUNOLOGY; v. 11, OCT 28 2020. |
| Citações Web of Science: | 0 |
| Resumo | |
Crotoxin (CTX), the main neurotoxin from Crotalus durissus terrificus snake venom, has anti-inflammatory, immunomodulatory and antinociceptive activities. However, the CTX-induced toxicity may compromise its use. Under this scenario, the use of nanoparticle such as nanostructured mesoporous silica (SBA-15) as a carrier might become a feasible approach to improve CTX safety. Here, we determined the benefits of SBA-15 on CTX-related neuroinflammatory and immunomodulatory properties during experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis that replicates several histopathological and immunological features observed in humans. We showed that a single administration of CTX:SBA-15 (54 mu g/kg) was more effective in reducing pain and ameliorated the clinical score (motor impairment) in EAE animals compared to the CTX-treated EAE group; therefore, improving the disease outcome. Of interest, CTX:SBA-15, but not unconjugated CTX, prevented EAE-induced atrophy and loss of muscle function. Further supporting an immune mechanism, CTX:SBA-15 treatment reduced both recruitment and proliferation of peripheral Th17 cells as well as diminished IL-17 expression and glial cells activation in the spinal cord in EAE animals when compared with CTX-treated EAE group. Finally, CTX:SBA-15, but not unconjugated CTX, prevented the EAE-induced cell infiltration in the CNS. These results provide evidence that SBA-15 maximizes the immunomodulatory and anti-inflammatory effects of CTX in an EAE model; therefore, suggesting that SBA-15 has the potential to improve CTX effectiveness in the treatment of MS. (AU) | |
| Processo FAPESP: | 17/17844-8 - Sílica nanoestruturada como veículo protetor de vacinas e biomoléculas |
| Beneficiário: | Osvaldo Augusto Brazil Esteves Sant'Anna |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 15/01254-1 - Avaliação do efeito da crotoxina conjugada à sílica nanoestruturada SBA-15 na dor crônica: aspectos imunomodulatórios, antiinflamatórios e antinociceptivos |
| Beneficiário: | Gisele Picolo |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 14/19397-0 - Avaliação do efeito da crotoxina conjugada à sílica nanoestruturada SBA-15 na dor crônica: aspectos imunomodulatórios, antiinflamatórios e antinociceptivos |
| Beneficiário: | Morena Brazil Martins Sant'Anna |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 13/07467-1 - CeTICS - Centro de Toxinas, Imuno-Resposta e Sinalização Celular |
| Beneficiário: | Hugo Aguirre Armelin |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |