| Texto completo | |
| Autor(es): |
de Freitas, Andre Teves A. G.
[1]
;
Pinho, Cristiane Figueiredo
[1]
;
Aquino, Ariana Musa
[1]
;
Domeniconi, Raquel Fantin
[1]
;
Justulin, Luis Antonio
[1]
;
Scarano, Wellerson Rodrigo
[1]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Sao Paulo State Univ UNESP, Inst Biosci, Dept Struct & Funct Biol, BR-18618970 Botucatu, SP - Brazil
Número total de Afiliações: 1
|
| Tipo de documento: | Artigo Científico |
| Fonte: | REPRODUCTIVE TOXICOLOGY; v. 96, p. 209-215, SEP 2020. |
| Citações Web of Science: | 1 |
| Resumo | |
This study was performed to evaluate the effect of monobutyl phthalate (MBP) on GPR30-activated pathways in Sertoli cells. Additionally, we tested if GIM-1 (Panax ginseng metabolite) modulates MBP action. Human Sertoli cells (HSeC lineage) were exposed to MBP and/or GIM-1 for 30 min, 1, 12, and 48 h. Four experimental treatments were performed: control (DEMEM/F12 medium), MBP, GIM-1, and MBP + GIM-1. The results indicate that MBP activates GPR30, PKA, Src, EGFR, and the ERK1/2 proteins, while GIM-1 inhibits PKA, Src, ERK1/2, and the AKT pathway. MBP also enhances Cofilin expression, decreasing F-actin intensity on the cell surface in a short time. The combined exposure demonstrated a functional antagonism between compounds. Collectively, these data show that MBP activates GPR30 in Sertoli cells, and GIM-1 modulates this response, playing a protective role in Sertoli cells exposed to MBP. (AU) | |
| Processo FAPESP: | 12/00253-3 - Análise do padrão de metilação das regiões controladoras de imprinting dos genes H19 e IGF2 e da resposta androgênica em células de Sertoli humanas expostas ao TCDD (2,3,7,8-tetraclorodibenzeno-p-dioxina) |
| Beneficiário: | Wellerson Rodrigo Scarano |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |