Panax ginseng metabolite (GIM-1) modulates the effects of monobutyl phthalate (MBP) on the GPR30/GPER1 canonical pathway in human Sertoli cells

Author(s)

de Freitas, Andre Teves A. G. ^[1] ; Pinho, Cristiane Figueiredo ^[1] ; Aquino, Ariana Musa ^[1] ; Domeniconi, Raquel Fantin ^[1] ; Justulin, Luis Antonio ^[1] ; Scarano, Wellerson Rodrigo ^[1]

Affiliation

^[1] Sao Paulo State Univ UNESP, Inst Biosci, Dept Struct & Funct Biol, BR-18618970 Botucatu, SP - Brazil

• Document type: Journal article
• Source: REPRODUCTIVE TOXICOLOGY; v. 96, p. 209-215, SEP 2020.
• Web of Science Citations: 1

Abstract

This study was performed to evaluate the effect of monobutyl phthalate (MBP) on GPR30-activated pathways in Sertoli cells. Additionally, we tested if GIM-1 (Panax ginseng metabolite) modulates MBP action. Human Sertoli cells (HSeC lineage) were exposed to MBP and/or GIM-1 for 30 min, 1, 12, and 48 h. Four experimental treatments were performed: control (DEMEM/F12 medium), MBP, GIM-1, and MBP + GIM-1. The results indicate that MBP activates GPR30, PKA, Src, EGFR, and the ERK1/2 proteins, while GIM-1 inhibits PKA, Src, ERK1/2, and the AKT pathway. MBP also enhances Cofilin expression, decreasing F-actin intensity on the cell surface in a short time. The combined exposure demonstrated a functional antagonism between compounds. Collectively, these data show that MBP activates GPR30 in Sertoli cells, and GIM-1 modulates this response, playing a protective role in Sertoli cells exposed to MBP. (AU)

• FAPESP's process: 12/00253-3 - Methylation pattern of the imprinting controlling regions and expression of the H19 and IGF-2 genes and androgenic response in human Sertoli cells after exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TDCC)
• Grantee: Wellerson Rodrigo Scarano
• Support Opportunities: Regular Research Grants