Acute effects of fatty acids on autophagy in NPY neurones

Autor(es):Mostrar menos -

Reginato, Andressa ^{[1, 2]} ; Siqueira, Beatriz Piatezzi ^{[1, 2]} ; Miyamoto, Josiane erica ^{[1, 2]} ; Portovedo, Mariana ^{[1, 2]} ; Costa, Suleyma de Oliveira ^{[1, 2]} ; de Fante, Thais ^{[1, 2]} ; Rodrigues, Hosana Gomes ^[1] ; Ignacio-Souza, Leticia Martins ^{[1, 2]} ; Torsoni, Marcio Alberto ^{[1, 2]} ; Torsoni, Adriana Souza ^[2] ; Le Stunff, Herve ^[3] ; Belsham, Denise D. ^[4] ; Milanski, Marciane ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Fac Appl Sci, Campinas, SP - Brazil

^[2] Univ Estadual Campinas, UNICAMP, Obes & Comorbid Res Ctr, Campinas - Brazil

^[3] Univ Paris Saclay, Neurosc Inst, Paris - France

^[4] Univ Toronto, Dept Physiol ObGyn & Med, Toronto, ON - Canada

• Tipo de documento: Artigo Científico
• Fonte: Journal of Neuroendocrinology; v. 32, n. 10 OCT 2020.
• Citações Web of Science: 0

Resumo

High-fat diet (HFD) feeding is deleterious to hypothalamic tissue, leading to inflammation and lipotoxicity, as well as contributing to central insulin resistance. Autophagy is a process that restores cellular homeostasis by degrading malfunctioning organelles and proteins. Chronic HFD-feeding down-regulates hypothalamic autophagy. However, the effects of short-term HFD-feeding and the saturated fatty acid palmitate (PA) on hypothalamic autophagy and in neurones that express neuropeptide Y (NPY) and agouti-related peptide remains unknown. Therefore, we assessed hypothalamic autophagy after 1 and 3 days of HFD-feeding. We also injected PA i.c.v and analysed the modulation of autophagy in hypothalamic tissue. Both interventions resulted in changes in autophagy-related gene profiles without significant differences in protein content of p62 and LC3B-II, markers of the autophagy pathway. When we assessed native NPY neurones in brain slices from PA-treated animals, we observed increased levels of Atg7 and LC3B protein in response to PA treatment, indicating the induction of autophagy. We then tested the direct effects of fatty acids using the immortalised hypothalamic NPY-expressing neuronal cell model mHypoE-46. We found that PA, but not palmitoleate (PO) (a monounsaturated fatty acid), was able to induce autophagy. Co-treatment with PA and PO was able to block the PA-mediated induction of autophagy, as assessed by flow cytometry. When the de novo ceramide synthesis pathway was blocked with myriocin pre-treatment, we observed a decrease in PA-mediated induction of autophagy, although there was no change with the toll-like receptor 4 inhibitor, TAK-242. Taken together, these findings provide evidence that saturated and unsaturated fatty acids can differentially regulate hypothalamic autophagy and that ceramide synthesis may be an important mediator of those effects. Understanding the mechanisms by which dietary fats affect autophagy in neurones involved in the control of energy homeostasis will provide potential new pathways for targeting and containing the obesity epidemic. (AU)

• Processo FAPESP: 15/25710-6 - Avaliação de autofagia hipotalâmica em resposta ao tratamento com ácido graxo saturado in vitro e in vivo: relação com vias inflamatórias.
• Beneficiário: Andressa Reginato
• Modalidade de apoio: Bolsas no Brasil - Doutorado