Amphiphilic copolymer aqueous solutions with cholinium ionic liquids as adjuvants: New insights into determination of binodal curves and phase-separation mechanisms

Autor(es)

Kurnik, Isabelle S. ^[1] ; Mussagy, Cassamo U. ^[1] ; Pereira, Jorge F. B. ^{[1, 2]} ; Lopes, Andre M. ^[3]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Engn Bioproc & Biotechnol, Araraquara, SP - Brazil

^[2] Univ Coimbra, CIEPQPF, Dept Chem Engn, Rua Silvio Lime, Polo 2 Pinhal de Marrocos, P-3030790 Coimbra - Portugal

^[3] Univ Estadual Campinas, Fac Pharmaceut Sci, Campinas - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF MOLECULAR LIQUIDS; v. 318, NOV 15 2020.
• Citações Web of Science: 0

Resumo

Aqueous two-phase micellar systems (ATPMS) are more than simple amphiphilic copolymer or surfactant aqueous solutions since due to the formation of biphasic regimes, at certain temperatures, these can be successfully used for the separation, encapsulation, and formulation of several bio-based compounds. The recent use of ionic liquids (ILs) as adjuvants in the formation of ATPMS have enlarged its ``working window{''}, but the measurement of respective binodal curves is still very empiric and dependent of operator accuracy through the visual determination of cloud-point temperature. In this work, the range of ATPMS using ILs as adjuvants was extended, providing more insights about their phase formation mechanisms, as well as into determination of doud-point values of the lower critical solution temperature (LOST)-type demixing transition by using the new temperature modulated optical refractometry approach. The binodal curves of ATPMS composed of Pluronic (R) L35/PBS buffer at pH 7.4 + cholinium IL were determined, by using two different methods, i.e.: cloud-point temperature (T-CP) visual determination versus temperature modulated optical refractometry (TMOR). TMOR method was more sensitive to the determination of biphasic transition, particularly, in highly diluted solutions (< 3.0 wt% of Pluronic L35). The results also established that the addition of cholinium ILs decreased significantly the phase separation temperatures of Pluronic L35/PBS, with an increase of biphasic region (viz. lower of Tcp values) as a function of the relative hydrophobicity of the IL anion, i.e.: {[}Hex](-) > {[}But](-) > {[}Pro](-) > {[}Ac](-) > Cl-. The use of cholinium ILs as adjuvants is particularly important to provide mild conditions for ATPMS-based separation and encapsulation strategies, while TMOR-based methods allow an improvement of phase separation determination accuracy. (C) 2020 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 14/16424-7 - Otimização e aumento de escala do processo de extração líquido-líquido com líquidos iônicos (LIs) como ferramenta de separação sustentável do biofármaco antileucêmico L-asparaginase (ASPase)
• Beneficiário: Jorge Pereira
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 18/05111-9 - Sistemas micelares de duas fases aquosas baseados em líquidos iônicos como plataformas para a encapsulação de curcumina em micelas poliméricas
• Beneficiário: Isabelle Souza Kurnik
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 19/05624-9 - Estudos de encapsulação e liberação in vitro de curcumina em micelas poliméricas compostas por copolímeros tribloco PEO-PPO-PEO
• Beneficiário: Isabelle Souza Kurnik
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Iniciação Científica
• Processo FAPESP: 17/10789-1 - Terapia combinatória utilizando polimerossomos decorados com transferrina e incorporados em hidrogéis de quitosana como smart drug delivery systems para células tumorais de melanoma
• Beneficiário: André Moreni Lopes
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 18/10799-0 - Terapia combinatória utilizando polimerossomos decorados com transferrina e incorporados em hidrogéis de quitosana como smart drug delivery systems para células tumorais de melanoma
• Beneficiário: André Moreni Lopes
• Modalidade de apoio: Bolsas no Brasil - Jovens Pesquisadores
• Processo FAPESP: 19/08549-8 - Polimerossomos decorados com transferrina e avaliação da sua estabilidade e comportamento de liberação de fármacos
• Beneficiário: Mayra Caroline Cunha Câmara
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica