Viral metagenomics in Brazilian multiply transfused patients with sickle cell disease as an indicator for blood transfusion safety

Autor(es)

Nunes Valenca, I ^{[1, 2]} ; Silva-Pinto, A. C. ^[2] ; Araujo da Silva Junior, W. ^[3] ; Covas, D. Tadeu ^[2] ; Kashima, S. ^[2] ; Nanev Slavov, S. ^{[4, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Postgrad Program Clin Oncol Stem Cells & Cell The, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Blood Ctr Ribeirao Preto, Rua Tenente Catao Roxo 2501, BR-14051140 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Internal Med, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: TRANSFUSION CLINIQUE ET BIOLOGIQUE; v. 27, n. 4, p. 237-242, NOV 2020.
• Citações Web of Science: 0

Resumo

Background and aim. - Patients with sickle cell disease (SCD) are submitted to multiple transfusions in order to increase the oxygen capacity of the blood, decrease blood viscosity, and suppress the sickling of the cells. Multiply transfused patients with SCD represent significant risk of acquiring parenterally transmitted infections. The analysis of the virome profile of high-risk multiply transfused patients with SCD can reveal the presence of parenterally transmitted viruses and therefore be used an indirect approach for evaluation of blood transfusion safety. Materials and methods. - Blood samples were collected from 45 patients with SCD receiving multiple transfusions and analyzed by metagenomic analyses. The samples were assembled in pools f which were submitted to nucleic acids extraction and sequencing by Illumina NextSeq 550 equipment. For bioinformatic analysis, we used a specific in-house developed pipeline specialized in identification of emerging viruses. Results. - The virome composition of SCD patients revealed the presence of commensal viruses represented by anelloviruses and Human Pegivirus-1 (HPgV-1, GB virus C). Contaminant viral sequences belonging to human lentiviruses (rev, env genes), cytomegalovirus and murine leukemia virus were also identified and are attributed to vectors used in the laboratory practice. No novel or unsuspected pathogenic viruses were identified. Conclusion. - This study evaluates for the first time the virome of multiply transfused patients with SCD. Exclusively genetic material of commensal viruses was annotated. Therefore, we believe that viral metagenomics applied in patients with high risk for acquiring parenterally transmitted infections can serve as a direct indicator for evaluation of transfusion safety. (C) 2020 Societe francaise de transfusion sanguine (SFTS). Published by Elsevier Masson SAS. All rights reserved. (AU)

• Processo FAPESP: 18/22009-3 - Aditividades de suporte técnico na coleta de amostras e técnicas moleculares em pacientes com anemia falciforme submetidos a múltiplas transfusões
• Beneficiário: Roberta Maraninchi Silveira
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 17/23205-8 - Avaliação do impacto de vírus emergentes e re-emergentes em hemoterapia e transplante de células-tronco por meio de técnicas moleculares avançadas
• Beneficiário: Svetoslav Nanev Slavov
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 19/07861-8 - Desenvolvimento de um pipeline de bioinformática para identificação de doenças infecciosas emergentes em pacientes em regime de transfusão crônica
• Beneficiário: Ian Nunes Valença
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 18/15826-5 - Avaliação do impacto de vírus emergentes e re-emergentes em hemoterapia e transplante de células-tronco por meio de técnicas moleculares avançadas
• Beneficiário: Svetoslav Nanev Slavov
• Modalidade de apoio: Bolsas no Brasil - Jovens Pesquisadores
• Processo FAPESP: 19/08528-0 - Aplicação de métodos de bioinformática para identificação de infecções virais com impacto em hemoterapia
• Beneficiário: Rafael dos Santos Bezerra
• Modalidade de apoio: Bolsas no Brasil - Mestrado