Molecular Tools for Detection and Identification of Paracoccidioides Species: Current Status and Future Perspectives

Autor(es)

Pinheiro, Breno Goncalves ^[1] ; Hahn, Rosane Christine ^{[2, 3]} ; de Camargo, Zoilo Pires ^{[1, 4]} ; Rodrigues, Anderson Messias ^[1]

Afiliação do(s) autor(es)

^[1] Fed Univ Sao Paulo UNIFESP, Dept Microbiol Immunol & Parasitol, Lab Emerging Fungal Pathogens, Discipline Cellular Biol, BR-04023062 Sao Paulo - Brazil

^[2] Univ Fed Mato Grosso, Fac Med, Lab Mycol Res, BR-78060900 Cuiaba, Mato Grosso - Brazil

^[3] Univ Fed Mato Grosso, Julio Muller Univ Hosp, BR-78048902 Mato Grosso - Brazil

^[4] Fed Univ Sao Paulo UNIFESP, Dept Med, Discipline Infect Dis, BR-04023062 Sao Paulo - Brazil

• Tipo de documento: Artigo de Revisão
• Fonte: JOURNAL OF FUNGI; v. 6, n. 4 DEC 2020.
• Citações Web of Science: 1

Resumo

Paracoccidioidomycosis (PCM) is a mycotic disease caused by the Paracoccidioides species, a group of thermally dimorphic fungi that grow in mycelial form at 25 degrees C and as budding yeasts when cultured at 37 degrees C or when parasitizing the host tissues. PCM occurs in a large area of Latin America, and the most critical regions of endemicity are in Brazil, Colombia, and Venezuela. The clinical diagnosis of PCM needs to be confirmed through laboratory tests. Although classical laboratory techniques provide valuable information due to the presence of pathognomonic forms of Paracoccidioides spp., nucleic acid-based diagnostics gradually are replacing or complementing culture-based, biochemical, and immunological assays in routine microbiology laboratory practice. Recently, taxonomic changes driven by whole-genomic sequencing of Paracoccidioides have highlighted the need to recognize species boundaries, which could better ascertain Paracoccidioides taxonomy. In this scenario, classical laboratory techniques do not have significant discriminatory power over cryptic agents. On the other hand, several PCR-based methods can detect polymorphisms in Paracoccidioides DNA and thus support species identification. This review is focused on the recent achievements in molecular diagnostics of paracoccidioidomycosis, including the main advantages and pitfalls related to each technique. We discuss these breakthroughs in light of taxonomic changes in the Paracoccidioides genus. (AU)

• Processo FAPESP: 17/27265-5 - Epidemiologia molecular e perspectivas genômicas na evolução e propagação de patógenos fúngicos emergentes
• Beneficiário: Anderson Messias Rodrigues
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 18/21460-3 - Estudo de diferentes preparações antigênicas de Paracoccidioides lutzii para a padronização do teste de ELISA como auxílio no diagnóstico da paracoccidioidomicose por P. lutzii
• Beneficiário: Zoilo Pires de Camargo
• Modalidade de apoio: Auxílio à Pesquisa - Regular