A Representative GIIA Phospholipase A(2) Activates Preadipocytes to Produce Inflammatory Mediators Implicated in Obesity Development

Autor(es)

Leiguez, Elbio ^[1] ; Motta, Priscila ^[1] ; Maia Marques, Rodrigo ^[1] ; Lomonte, Bruno ^[2] ; Sampaio, Suely Vilela ^[3] ; Teixeira, Catarina ^[1]

Afiliação do(s) autor(es)

^[1] Inst Butantan, Lab Farmacol, BR-05503900 Sao Paulo - Brazil

^[2] Univ Costa Rica, Fac Microbiol, Inst Clodomiro Picado, San Jose 115012060 - Costa Rica

^[3] Univ Sao Paulo, Fac Ciencias Farmaceut Riberao Preto, BR-14040903 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOMOLECULES; v. 10, n. 12 DEC 2020.
• Citações Web of Science: 0

Resumo

Adipose tissue secretes proinflammatory mediators which promote systemic and adipose tissue inflammation seen in obesity. Group IIA (GIIA)-secreted phospholipase A(2) (sPLA(2)) enzymes are found to be elevated in plasma and adipose tissue from obese patients and are active during inflammation, generating proinflammatory mediators, including prostaglandin E-2 (PGE(2)). PGE(2) exerts anti-lipolytic actions and increases triacylglycerol levels in adipose tissue. However, the inflammatory actions of GIIA sPLA(2)s in adipose tissue cells and mechanisms leading to increased PGE(2) levels in these cells are unclear. This study investigates the ability of a representative GIIA sPLA(2), MT-III, to activate proinflammatory responses in preadipocytes, focusing on the biosynthesis of prostaglandins, adipocytokines and mechanisms involved in these effects. Our results showed that MT-III induced biosynthesis of PGE(2), PGI(2), MCP-1, IL-6 and gene expression of leptin and adiponectin in preadipocytes. The MT-III-induced PGE(2) biosynthesis was dependent on cytosolic PLA(2) (cPLA(2))-alpha, cyclooxygenases (COX)-1 and COX-2 pathways and regulated by a positive loop via the EP4 receptor. Moreover, MT-III upregulated COX-2 and microsomal prostaglandin synthase (mPGES)-1 protein expression. MCP-1 biosynthesis induced by MT-III was dependent on the EP4 receptor, while IL-6 biosynthesis was dependent on EP3 receptor engagement by PGE(2). These data highlight preadipocytes as targets for GIIA sPLA(2)s and provide insight into the roles played by this group of sPLA(2)s in obesity. (AU)

• Processo FAPESP: 11/23236-4 - Toxinas animais nativas e recombinantes: análise funcional, estrutural e molecular
• Beneficiário: Suely Vilela
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 15/24701-3 - Estudo dos efeitos de uma fosfolipase a2 de veneno ofidico em células do tecido adiposo: adipogênese e resposta inflamatória
• Beneficiário: Elbio Leiguez Junior
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado