| Texto completo | |
| Autor(es): |
Pereira, Marita Gimenez
[1]
;
de Almeida, Otavio Guilherme Goncalves
[1]
;
da Silva, Hevelin Regiane Augusto
[1]
;
Ishizawa, Marilia Harumi
[1]
;
De Martinis, Elaine Cristina Pereira
[1]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Ave Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil
Número total de Afiliações: 1
|
| Tipo de documento: | Artigo Científico |
| Fonte: | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY; v. 37, n. 2 FEB 2021. |
| Citações Web of Science: | 0 |
| Resumo | |
Spheroids of intestinal cells (Caco-2) were used to evaluate the adhesion/invasion ability of Listeria monocytogenes (pathogen) and Lactobacillus sakei 1 (potential probiotic). Besides, transcriptomic analyses of Caco-2 cells in three dimensional cultures were done, with the aim of revealing possible host-foodborne bacteria interactions. Result of adhesion assay for L. monocytogenes in Caco-2 spheroids was 22.86 +/- 0.33%, but it was stimulated in acidic pH (4.5) and by the presence of 2% sucrose (respectively, 32.56 +/- 1.35% and 33.25 +/- 1.26%). Conversely, the invasion rate of L. monocytogenes was lower at pH 4.5, in comparison with non-stressed controls (18.89 +/- 1.05% and 58.65 +/- 0.30%, respectively). L. sakei 1 adhered to Caco-2 tridimensional cell culture (27.30 +/- 2.64%), with no invasiveness. There were 19 and 21 genes down and upregulated, respectively, in tridimensional Caco-2 cells, upon infection with L. monocytogenes, which involved immunity, apoptosis; cytoprotective responses, cell signalling-regulatory pathways. It was evidenced despite activation or deactivation of several pathways in intestinal cells to counteract infection, the pathogen was able to hijack many host defense mechanisms. On the other hand, the probiotic candidate L. sakei 1 was correlated with decreased transcription of two genes in Caco-2 cells, though it stimulated the expression of 14 others, with diverse roles in immunity, apoptosis, cytoprotective response and cell signalling-regulatory pathways. Our data suggest the use of tridimensional cell culture to mimic the intestinal epithelium is a good model for gathering broad information on the putative mechanisms of interaction between host and bacteria of importance for food safety, which can serve as a basis for further in-depth investigation. (AU) | |
| Processo FAPESP: | 17/18928-0 - "Organóides intestinais obtidos in vitro por cultura de células tridimensional (3D) para estudos de virulência e/ou propriedades probióticas de bactérias e compostos de interesse em alimentos" |
| Beneficiário: | Elaine Cristina Pereira de Martinis |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 17/13759-6 - Prospecção metagenômica de bactérias relacionadas a quorum sensing durante a fermentação espontânea do cacau |
| Beneficiário: | Otávio Guilherme Gonçalves de Almeida |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |
| Processo FAPESP: | 16/16293-5 - Ferramentas omicas aplicadas a elucidação do papel da microbiota intestinal na remissão de Doença Inflamatória Intestinal baseada em dieta terapêutica |
| Beneficiário: | Elaine Cristina Pereira de Martinis |
| Modalidade de apoio: | Bolsas no Exterior - Pesquisa |