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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Studies on host-foodborne bacteria in intestinal three-dimensional cell culture model indicate possible mechanisms of interaction

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Author(s):
Pereira, Marita Gimenez [1] ; de Almeida, Otavio Guilherme Goncalves [1] ; da Silva, Hevelin Regiane Augusto [1] ; Ishizawa, Marilia Harumi [1] ; De Martinis, Elaine Cristina Pereira [1]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Ave Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY; v. 37, n. 2 FEB 2021.
Web of Science Citations: 0
Abstract

Spheroids of intestinal cells (Caco-2) were used to evaluate the adhesion/invasion ability of Listeria monocytogenes (pathogen) and Lactobacillus sakei 1 (potential probiotic). Besides, transcriptomic analyses of Caco-2 cells in three dimensional cultures were done, with the aim of revealing possible host-foodborne bacteria interactions. Result of adhesion assay for L. monocytogenes in Caco-2 spheroids was 22.86 +/- 0.33%, but it was stimulated in acidic pH (4.5) and by the presence of 2% sucrose (respectively, 32.56 +/- 1.35% and 33.25 +/- 1.26%). Conversely, the invasion rate of L. monocytogenes was lower at pH 4.5, in comparison with non-stressed controls (18.89 +/- 1.05% and 58.65 +/- 0.30%, respectively). L. sakei 1 adhered to Caco-2 tridimensional cell culture (27.30 +/- 2.64%), with no invasiveness. There were 19 and 21 genes down and upregulated, respectively, in tridimensional Caco-2 cells, upon infection with L. monocytogenes, which involved immunity, apoptosis; cytoprotective responses, cell signalling-regulatory pathways. It was evidenced despite activation or deactivation of several pathways in intestinal cells to counteract infection, the pathogen was able to hijack many host defense mechanisms. On the other hand, the probiotic candidate L. sakei 1 was correlated with decreased transcription of two genes in Caco-2 cells, though it stimulated the expression of 14 others, with diverse roles in immunity, apoptosis, cytoprotective response and cell signalling-regulatory pathways. Our data suggest the use of tridimensional cell culture to mimic the intestinal epithelium is a good model for gathering broad information on the putative mechanisms of interaction between host and bacteria of importance for food safety, which can serve as a basis for further in-depth investigation. (AU)

FAPESP's process: 17/18928-0 - Organoid-like intestinal tridimensional (3D) culture for studies on virulence and/or probiotic properties of bacteria and food related compounds
Grantee:Elaine Cristina Pereira de Martinis
Support type: Regular Research Grants
FAPESP's process: 17/13759-6 - Metagenomic prospection of quorum sensing related bacteria during spontaneous cocoa beans fermentation
Grantee:Otávio Guilherme Gonçalves de Almeida
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 16/16293-5 - Omic tools applied to elucidation of the role of intestinal microbiota in the remission of Inflammatory Bowel Disease (IBD) based on therapeutic diet
Grantee:Elaine Cristina Pereira de Martinis
Support type: Scholarships abroad - Research