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Role of EspFu protein on atypical enteropathogenic Escherichia coli

Grant number: 13/17403-0
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): November 01, 2013
Effective date (End): September 30, 2017
Field of knowledge:Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
Principal Investigator:Waldir Pereira Elias Junior
Grantee:Fernando Henrique Martins
Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
Associated scholarship(s):16/08401-2 - Dynamics of pedestal formation by atypical Enteropathogenic Escherichia coli strains displaying distinct actin polymerization pathways, BE.EP.DR


Atypical enteropathogenic Escherichia coli (aEPEC) is an important etiological agent of infantile diarrhea worldwide, including Brazil. The central mechanism of aEPEC pathogenesis is the ability to cause attaching-effacing lesions on intestinal epithelium, a property triggered by proteins encoded on a pathogenicity island called locus of enterocyte effacement. However, some strains produce an important non-LEE-encoded effector protein named EspFu, which is involved in the formation of A/E lesions and colonization of the intestinal epithelium, and thus may contribute to the pathogenicity of aEPEC. According to this, a study recently conducted in our laboratory demonstrated the participation of EspFu on LEE gene expression and adhesion of a nonadherent aEPEC isolate, suggesting a possible new role played by this protein. The espFu gene is commonly detected among pathogenic aEPEC O55:H7 strains, indicating that this protein may play an important role on pathogenesis of this group. Thus, the main objective of this work is to investigate the role of EspFu protein on aEPEC pathogenesis, using an espFu-positive O55:H7 strain as study model. Therefore, it will be examined the temporal expression and production of EspFu in aEPEC O55:H7 during the infection of intestinal epithelial cells. Further, an aEPEC espFu mutant will be compared to wild type and espFu-complemented mutant strains with respect to motility, biofilm formation, expression of the LEE operons, interaction with cultured epithelial cells and intestinal colonization of streptomycin-treated mice. Thus, we intend to better understand the contribution of EspFu to aEPEC pathogenesis, as well as to describe a new role played by this protein on bacterial cell compartment.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MARTINS, FERNANDO H.; NEPOMUCENO, ROBERTO; PIAZZA, ROXANE M. F.; ELIAS, WALDIR P.. Phylogenetic distribution of tir-cytoskeleton coupling protein (tccP and tccP2) genes in atypical enteropathogenic Escherichia coli. FEMS Microbiology Letters, v. 364, n. 11, . (13/17403-0)
MARTINS, FERNANDO H.; KUMAR, ASHWANI; ABE, CECILIA M.; CARVALHO, ENEAS; NISHIYAMA-JR, MILTON; XING, CHAO; SPERANDIO, VANESSA; ELIAS, WALDIR P.. EspFu-Mediated Actin Assembly Enhances Enteropathogenic Escherichia coli Adherence and Activates Host Cell Inflammatory Signaling Pathways. MBIO, v. 11, n. 2, . (11/14103-0, 16/08401-2, 13/17403-0)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
MARTINS, Fernando Henrique. Role of EspFU protein in atypical enteropathogenic Escherichia coli.. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.

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