Silver nanoparticles stabilized with propolis show reduced toxicity and potential activity against fungal infections

Autor(es)

Kischkel, Brenda ^{[1, 2]} ; de Castilho, Pamella F. ^[3] ; de Oliveira, Kelly M. P. ^[3] ; Rezende, Pamela S. T. ^[1] ; Bruschi, Marcos L. ^[4] ; Svidzinski, Terezinha I. E. ^[1] ; Negri, Melyssa ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Maringa, Clin Anal Dept, Ave Colombo 5790, Maringa, PR - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Ave Prof Lineu Prestes 1374, Sao Paulo, SP - Brazil

^[3] Fundacao Univ Fed Grande Dourados, Fac Biol & Environm Sci, BR-1761 Dourados, MS - Brazil

^[4] Univ Estadual Maringa, Dept Pharm, Ave Colombo 5790, Maringa, PR - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FUTURE MICROBIOLOGY; v. 15, n. 7, p. 521-539, MAY 2020.
• Citações Web of Science: 3

Resumo

Aim: Elucidate the antifungal efficacy of biologically synthesized silver nanoparticles with ethanolic propolis extract (AgNPs PE) against the planktonic forms and biofilms of clinically important fungi. Materials \& methods: AgNPs were synthesized, characterized and evaluated for cytotoxicity, mutagenicity and antimicrobial activity. Results: AgNPs PE displayed a colloidal appearance, good stability and size of 2.0-40.0 nm. AgNPs PE demonstrated lower cytotoxicity and nonmutagenic potential. In addition, AgNPs PE displayed antifungal properties against all tested isolates, inhibiting growth at concentrations lower than the cytotoxic effect. Mature biofilms treated for 48 h with AgNPs PE showed significant reduction of viable cells, metabolic activity and total biomass. Conclusion: This is the first time that AgNPs have been synthesized from an ethanolic extract of propolis only, proving antifungal, antibiofilm, atoxic and nonmutagenic properties. (AU)

• Processo FAPESP: 18/26402-1 - Prospecção de novos epítopos com potencial vacinal no controle da infecção experimental por Histoplasma capsulatum
• Beneficiário: Brenda Kischkel
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto