| Texto completo | |
| Autor(es): |
Antunes, Natalicia J.
[1, 2, 3]
;
Kipper, Karin
[4, 3]
;
Couchman, Lewis
[5, 3]
;
Duncan, Marie-Anne
[3]
;
Holt, David W.
[3]
;
De Nucci, Gilberto
[1]
;
Johnston, Atholl
[2, 3]
Número total de Autores: 7
|
| Afiliação do(s) autor(es): | [1] State Univ Campinas UNICAMP, Fac Med Sci, Dept Pharmacol, Campinas, SP - Brazil
[2] Queen Mary Univ London, Barts & London Sch Med & Dent, Clin Pharmacol, William Harvey Res Inst, Charterhouse Sq, London EC1M 6BQ - England
[3] St Georges Univ London, Analyt Serv Int ASI Ltd, London - England
[4] Univ Tartu, Inst Chem, Tartu - Estonia
[5] Kings Coll London, Pharmaceut Sci Clin Acad Grp, London - England
Número total de Afiliações: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BIOMEDICAL CHROMATOGRAPHY; v. 35, n. 6 FEB 2021. |
| Citações Web of Science: | 1 |
| Resumo | |
The aim of this study was to develop and validate a UHPLC-MS/MS assay to quantify cyclosporin (CYC), tacrolimus (TAC), sirolimus (SIR) and everolimus (EVE) in human whole blood for therapeutic drug monitoring. Analytes were extracted from 50 mu L human whole blood by protein precipitation. The separation of the drugs was performed on an Acquity UPLC BEH C18 column. Analytes were eluted with a mobile phase consisting of 2 mM ammonium acetate with 0.1% formic acid (v/v) in deionised water and 2 mM ammonium acetate with 0.1% formic acid (v/v) in methanol at a flow rate of 300 mu L/min in gradient elution. The method performance was evaluated by analysing patient blood samples and/or external quality control samples {[}proficiency testing (PT) scheme]. The method was linear from 23.75 to 1094.0, 1.3 to 42.4, 1.3 to 47.0 and 1.2-41.6 mu g/mL for CYC, TAC, SIR and EVE, respectively. The within- and between-assay reproducibility results were < 11%. Results from PT and patient sample quantification were comparable to those obtained previously by an in-house validated method using protein precipitation and liquid-liquid extraction. This method showed good analytical performance for quantifying CYC, TAC, SIR and EVE in whole blood over their respective calibration ranges. (AU) | |
| Processo FAPESP: | 16/22506-1 - Metabolismo do dapaconazol |
| Beneficiário: | Natalícia de Jesus Antunes |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |