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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Simultaneous quantification of cyclosporin, tacrolimus, sirolimus and everolimus in whole blood by UHPLC-MS/MS for therapeutic drug monitoring

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Author(s):
Antunes, Natalicia J. [1, 2, 3] ; Kipper, Karin [4, 3] ; Couchman, Lewis [5, 3] ; Duncan, Marie-Anne [3] ; Holt, David W. [3] ; De Nucci, Gilberto [1] ; Johnston, Atholl [2, 3]
Total Authors: 7
Affiliation:
[1] State Univ Campinas UNICAMP, Fac Med Sci, Dept Pharmacol, Campinas, SP - Brazil
[2] Queen Mary Univ London, Barts & London Sch Med & Dent, Clin Pharmacol, William Harvey Res Inst, Charterhouse Sq, London EC1M 6BQ - England
[3] St Georges Univ London, Analyt Serv Int ASI Ltd, London - England
[4] Univ Tartu, Inst Chem, Tartu - Estonia
[5] Kings Coll London, Pharmaceut Sci Clin Acad Grp, London - England
Total Affiliations: 5
Document type: Journal article
Source: BIOMEDICAL CHROMATOGRAPHY; v. 35, n. 6 FEB 2021.
Web of Science Citations: 1
Abstract

The aim of this study was to develop and validate a UHPLC-MS/MS assay to quantify cyclosporin (CYC), tacrolimus (TAC), sirolimus (SIR) and everolimus (EVE) in human whole blood for therapeutic drug monitoring. Analytes were extracted from 50 mu L human whole blood by protein precipitation. The separation of the drugs was performed on an Acquity UPLC BEH C18 column. Analytes were eluted with a mobile phase consisting of 2 mM ammonium acetate with 0.1% formic acid (v/v) in deionised water and 2 mM ammonium acetate with 0.1% formic acid (v/v) in methanol at a flow rate of 300 mu L/min in gradient elution. The method performance was evaluated by analysing patient blood samples and/or external quality control samples {[}proficiency testing (PT) scheme]. The method was linear from 23.75 to 1094.0, 1.3 to 42.4, 1.3 to 47.0 and 1.2-41.6 mu g/mL for CYC, TAC, SIR and EVE, respectively. The within- and between-assay reproducibility results were < 11%. Results from PT and patient sample quantification were comparable to those obtained previously by an in-house validated method using protein precipitation and liquid-liquid extraction. This method showed good analytical performance for quantifying CYC, TAC, SIR and EVE in whole blood over their respective calibration ranges. (AU)

FAPESP's process: 16/22506-1 - Metabolism of dapaconazole
Grantee:Natalícia de Jesus Antunes
Support Opportunities: Scholarships in Brazil - Post-Doctoral