Therapeutic drug monitoring (TDM) of immunosuppressive drugs is used to improve the immunosuppressive effect while minimizing the toxicity related to exposition to high serum levels. Although TDM is widely used in clinical practice, a significant number of kidney transplant recipients have acute allograft rejection in the first year after transplantation. To improve the use of immunosuppressive drugs, new approaches of TDM have been developed. Monitoring drug concentrations at lymphocytes of peripheral blood is considering promising because it indicates the availability of the drug directly in the target sites of immunosuppression.The ultra high performance liquid chromatography - mass spectrometry (UPLC-MS/MS) is an appropriate tool to monitor intra-lymphocyte immunosuppressive drugs concentrationsbecause the technic is highly precise performing quantification of drugs in low concentrations is minimal volume of biological samples. The aim of this study are to optimize and validate the UPLC-MS/MS assay for tacrolimus quantification separately in PBMC, TCD4+ and B peripheral blood lymphocytes.
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