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Validation of an ultra high performance liquid chromatography - mass spectrometry assay for tacrolimus quantification in matrices of peripheral blood t (CD4+) and b lymphocytes

Grant number: 16/10043-7
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): August 01, 2016
Effective date (End): July 31, 2017
Field of knowledge:Health Sciences - Medicine - Medical Clinics
Principal researcher:Nelson Zocoler Galante
Grantee:Nayara Duarte de Oliveira
Home Institution: Hospital das Clínicas da Faculdade de Medicina da USP (HCFMUSP). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil


Therapeutic drug monitoring (TDM) of immunosuppressive drugs is used to improve the immunosuppressive effect while minimizing the toxicity related to exposition to high serum levels. Although TDM is widely used in clinical practice, a significant number of kidney transplant recipients have acute allograft rejection in the first year after transplantation. To improve the use of immunosuppressive drugs, new approaches of TDM have been developed. Monitoring drug concentrations at lymphocytes of peripheral blood is considering promising because it indicates the availability of the drug directly in the target sites of immunosuppression.The ultra high performance liquid chromatography - mass spectrometry (UPLC-MS/MS) is an appropriate tool to monitor intra-lymphocyte immunosuppressive drugs concentrationsbecause the technic is highly precise performing quantification of drugs in low concentrations is minimal volume of biological samples. The aim of this study are to optimize and validate the UPLC-MS/MS assay for tacrolimus quantification separately in PBMC, TCD4+ and B peripheral blood lymphocytes.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ROMANO, PASCHOALINA; FERNANDES, MARIA DA LUZ; REZENDE EBNER, PERSIO DE ALMEIDA; DE OLIVEIRA, NAYARA DUARTE; OKUDA, LARISSA MITSUE; AGENA, FABIANA; MENDES, MARIA ELIZABETE; SUMITA, NAIRO MASSAKAZU; COELHO, VERONICA; DAVID-NETO, ELIAS; GALANTE, NELSON ZOCOLER. UPLC-MS/MS assay validation for tacrolimus quantitative determination in peripheral blood T CD4+and B CD19+lymphocytes. Journal of Pharmaceutical and Biomedical Analysis, v. 152, p. 306-314, APR 15 2018. Web of Science Citations: 3.

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