Goncalves, Elizamara S.
Oliveira, Victoria R. S.
Toniolo, Elaine F.
Alves, Adilson S.
de Andrade, Daniel C.
Teixeira, Manoel J.
Britto, Luiz R. G.
Dale, Camila S.
Número total de Autores: 12
Afiliação do(s) autor(es):
 Univ Sao Paulo, Dept Anat, Inst Biomed Sci, Av Prof Lineu Prestes 2415, ICB 3, Cidade Univ, BR-05508900 Sao Paulo, SP - Brazil
 Univ Cidade Sao Paulo, Ctr Res Neurosci, R Cesario Galero 448-475 Tatuape, BR-03071000 Sao Paulo, SP - Brazil
 Univ Sao Paulo, Dept Physiol & Biophys, Inst Biomed Sci, BR-05508000 Sao Paulo, SP - Brazil
 Inst Butantan, Lab Biochem & Biophys, Sao Paulo - Brazil
 Cent Inst, Dept Neurol, Av Dr Eneas Carvalho Aguiar 255, 5th Floor, Room 50, BR-05403900 Sao Paulo, SP - Brazil
 Univ Sao Paulo, Inst Canc Octavio Friers Oliveira, Sao Paulo - Brazil
 Univ Toronto, Harquail Ctr Neuromodulat, Sunnybrook Res Inst, Toronto, ON M4N 3M5 - Canada
 Univ Sao Paulo, Med Sch, Dept Surg Tech, Av Dr Arnaldo 455, BR-01246903 Sao Paulo, SP - Brazil
Número total de Afiliações: 8
Tipo de documento:
MAR 1 2021.
Citações Web of Science:
The insula has emerged as a critical target for electrical stimulation since it influences pathological pain states. We investigated the effects of repetitive electrical stimulation of the insular cortex (ESI) on mechanical nociception, and general locomotor activity in rats subjected to chronic constriction injury (CCI) of the sciatic nerve. We also studied neuroplastic changes in central pain areas and the involvement of GABAergic signaling on ESI effects. CCI rats had electrodes implanted in the left agranular posterior insular cortex (pIC), and mechanical sensitivity was evaluated before and after one or five daily consecutive ESIs (15 min each, 60 Hz, 210 mu s, 1 V). Five ESIs (repetitive ESI) induced sustained mechanical antinociception from the first to the last behavioral assessment without interfering with locomotor activity. A marked increase in Fos immunoreactivity in pIC and a decrease in the anterior and mid-cingulate cortex, periaqueductal gray and hippocampus were noticed after five ESIs. The intrathecal administration of the GABA(A) receptor antagonist bicuculline methiodide reversed the stimulation-induced antinociception after five ESIs. ESI increased GAD65 levels in pIC but did not interfere with GABA, glutamate or glycine levels. No changes in GFAP immunoreactivity were found in this work. Altogether, the results indicate the efficacy of repetitive ESI for the treatment of experimental neuropathic pain and suggest a potential influence of pIC in regulating pain pathways partially through modulating GABAergic signaling. (AU)