Fed-batch production of Thermothelomyces thermophilus lignin peroxidase using a recombinant Aspergillus nidulans strain in stirred-tank bioreactor

Autor(es)

Liu, Enshi ^[1] ; Segato, Fernando ^[2] ; Wilkins, Mark R. ^{[3, 1, 4]}

Afiliação do(s) autor(es)

^[1] Univ Nebraska, Dept Biol Syst Engn, Lincoln, NE 68583 - USA

^[2] Univ Sao Paulo, Dept Biotechnol, Lorena, SP - Brazil

^[3] Univ Nebraska, Dept Food Sci & Technol, Lincoln, NE 68588 - USA

^[4] Univ Nebraska, Ind Agr Prod Ctr, Lincoln, NE 68583 - USA

• Tipo de documento: Artigo Científico
• Fonte: Bioresource Technology; v. 325, APR 2021.
• Citações Web of Science: 4

Resumo

Enzymatic lignin depolymerization is considered a favorable approach to utilize lignin due to the higher selectivity and less energy requirement when compared to thermochemical lignin valorization. Lignin peroxidase (LiP) is one of the key enzymes involved in lignin degradation and possesses high redox potential to oxidize non-phenolic structures and phenolic compounds in lignin. However, the production of LiP is mainly from white-rot fungi at small scales. It is critical to discover new LiP from other microorganisms and produce LiP at large scales. This study aims to produce a novel LiP originally from Thermothelomyces thermophiles using a recombinant Aspergillus nidulans strain. The LiP production medium was optimized, and different fed-batch strategies for LiP production were investigated to improve LiP activity, yield, and productivity. Results demonstrated that LiP production was enhanced by using multi-pulse fed-batch fermentation. A maximum LiP activity of 1,645 mU/L with a protein concentration of 0.26 g/L was achieved. (AU)

• Processo FAPESP: 14/18714-2 - Oxidação enzimática do bagaço de cana-de-açúcar: descoberta, caracterização e aplicação de novas enzimas oxidativas de carboidratos, voltadas para o desenvolvimento de um cell factory mais eficiente
• Beneficiário: Fernando Segato
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Jovens Pesquisadores
• Processo FAPESP: 17/50025-0 - Development of lignin active enzyme mixture to produce sugars from lignocellulosic biomass for production of fuels, chemicals and bio based materials
• Beneficiário: Fernando Segato
• Modalidade de apoio: Auxílio à Pesquisa - Regular