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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Fed-batch production of Thermothelomyces thermophilus lignin peroxidase using a recombinant Aspergillus nidulans strain in stirred-tank bioreactor

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Author(s):
Liu, Enshi [1] ; Segato, Fernando [2] ; Wilkins, Mark R. [3, 1, 4]
Total Authors: 3
Affiliation:
[1] Univ Nebraska, Dept Biol Syst Engn, Lincoln, NE 68583 - USA
[2] Univ Sao Paulo, Dept Biotechnol, Lorena, SP - Brazil
[3] Univ Nebraska, Dept Food Sci & Technol, Lincoln, NE 68588 - USA
[4] Univ Nebraska, Ind Agr Prod Ctr, Lincoln, NE 68583 - USA
Total Affiliations: 4
Document type: Journal article
Source: Bioresource Technology; v. 325, APR 2021.
Web of Science Citations: 0
Abstract

Enzymatic lignin depolymerization is considered a favorable approach to utilize lignin due to the higher selectivity and less energy requirement when compared to thermochemical lignin valorization. Lignin peroxidase (LiP) is one of the key enzymes involved in lignin degradation and possesses high redox potential to oxidize non-phenolic structures and phenolic compounds in lignin. However, the production of LiP is mainly from white-rot fungi at small scales. It is critical to discover new LiP from other microorganisms and produce LiP at large scales. This study aims to produce a novel LiP originally from Thermothelomyces thermophiles using a recombinant Aspergillus nidulans strain. The LiP production medium was optimized, and different fed-batch strategies for LiP production were investigated to improve LiP activity, yield, and productivity. Results demonstrated that LiP production was enhanced by using multi-pulse fed-batch fermentation. A maximum LiP activity of 1,645 mU/L with a protein concentration of 0.26 g/L was achieved. (AU)

FAPESP's process: 17/50025-0 - Development of lignin active enzyme mixture to produce sugars from lignocellulosic biomass for production of fuels, chemicals and bio based materials
Grantee:Fernando Segato
Support type: Regular Research Grants
FAPESP's process: 14/18714-2 - Enzymatic oxidation of sugarcane bagasse: discovery, characterization and new application of oxidative enzymes active in carbohydrates, applied to the enhancement of a fungal cell factory
Grantee:Fernando Segato
Support type: Program for Research on Bioenergy (BIOEN) - Young Investigators Grants