A novel method for DNA delivery into bacteria using cationic copolymers

Autor(es)

V.V. de Souza ; P.A.M. Vitale ; F.H. Florenzano ; R.K. Salinas ; I.M. Cuccovia

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Medical and Biological Research; v. 54, n. 5, p. -, 2021.
• Citações Web of Science: 0

Resumo

Amphiphilic copolymers have a wide variety of medical and biotechnological applications, including DNA transfection in eukaryotic cells. Still, no polymer-primed transfection of prokaryotic cells has been described. The reversible addition-fragmentation chain transfer (RAFT) polymer synthesis technique and the reversible deactivation radical polymerization variants allow the design of polymers with well-controlled molar mass, morphology, and hydrophilicity/hydrophobicity ratios. RAFT was used to synthesize two amphiphilic copolymers containing different ratios of the amphiphilic poly[2-(dimethyl-amino) ethyl methacrylate] and the hydrophobic poly [methyl methacrylate]. These copolymers bound to pUC-19 DNA and successfully transfected non-competent Escherichia coli DH5α, with transformation efficiency in the range of 103 colony-forming units per µg of plasmid DNA. These results demonstrate prokaryote transformation using polymers with controlled amphiphilic/hydrophobic ratios. (AU)

• Processo FAPESP: 16/07490-1 - Estudos estruturais acerca do trocador de Na+/Ca2+ e de proteínas do vírus Zika por espectroscopia de Ressonância Magnética Nuclear em solução
• Beneficiário: Roberto Kopke Salinas
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/08166-5 - Química em interfaces: interações de fármacos, peptídios e enzimas com membranas modelos
• Beneficiário: Iolanda Midea Cuccovia
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 17/05614-8 - Trocadores de Na+/Ca2+: relação entre estrutura e função
• Beneficiário: Phelipe Augusto Mariano Vitale
• Linha de fomento: Bolsas no Brasil - Doutorado Direto