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Autor(es):
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Arruda, Gian Lucas M.
[1]
;
Vigerelli, Hugo
[2]
;
Bufalo, Michelle C.
[3]
;
Longato, Giovanna B.
[4]
;
Veloso, Rodinei V.
[1]
;
Zambelli, Vanessa O.
[3]
;
Picolo, Gisele
[3]
;
Cury, Yara
[3]
;
Morandini, Andre C.
[5, 6]
;
Marques, Antonio Carlos
[5]
;
Sciani, Juliana Mozer
[1]
Número total de Autores: 11
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| Afiliação do(s) autor(es): | [1] Univ Sao Francisco, Lab Multidisciplinar Pesquisa, BR-12916900 Braganca Paulista, SP - Brazil
[2] Inst Butantan, Lab Genet, BR-05503900 Sao Paulo - Brazil
[3] Inst Butantan, Lab Dor & Sinalizacao, BR-05503900 Sao Paulo - Brazil
[4] Univ Sao Francisco, Lab Pesquisa Farmacol Mol & Compostos Bioat, BR-12916900 Braganca Paulista, SP - Brazil
[5] Univ Sao Paulo, Inst Biociencias, Dept Zool, BR-05508090 Sao Paulo - Brazil
[6] Univ Sao Paulo, Ctr Biol Marinha, BR-11612109 Sao Sebastiao - Brazil
Número total de Afiliações: 6
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| Tipo de documento: |
Artigo Científico
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| Fonte: |
BIOMED RESEARCH INTERNATIONAL;
v. 2021,
MAR 5 2021.
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| Citações Web of Science: |
0
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| Resumo |
Neurodegenerative diseases are one of the major causes of death worldwide, characterized by neurite atrophy, neuron apoptosis, and synapse loss. No effective treatment has been indicated for such diseases so far, and the search for new drugs is being increased in the last years. Animal venoms' secretion/venom can be an alternative for the discovery of new molecules, which could be the prototype for a new treatment. Here, we present the biochemical characterization and activity of the extract from the box jellyfish Chiropsalmus quadrumanus (Cq) on neurites. The Cq methanolic extract was obtained and incubated to human SH-SY5Y neurons, and neurite parameters were evaluated. The extract was tested in other cell types to check its cytotoxicity and was submitted to biochemical analysis by mass spectrometry in order to check its composition. We could verify that the Cq extract increased neurite outgrowth length and branching junctions, amplifying the contact between SH-SY5Y neurons, without affecting cell body and viability. The extract action was selective for neurons, as it did not cause any effects on other cell types, such as tumor line, nontumor line, and red blood cells. Moreover, mass spectrometry analysis revealed that there are no proteins but several low molecular mass compounds and peptides. Three peptides, characterized as cryptides, and 14 low molecular mass compounds were found to be related to cytoskeleton reorganization, cell membrane expansion, and antioxidant/neuroprotective activity, which act together to increase neuritogenesis. After this evaluation, we conclude that the Cq extract is a promising tool for neuronal connection recovery, an essential condition for the treatment of neurodegenerative diseases. (AU) |
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| Processo FAPESP: |
15/21007-9 - Reconhecendo a diversidade de águas-vivas (Medusozoa, Rhopaliophora)
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| Beneficiário: | André Carrara Morandini |
| Linha de fomento: |
Auxílio à Pesquisa - Regular
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| Processo FAPESP: |
16/06137-6 - Bioprospecção de compostos naturais e derivados bioativos com potencial atividade anticâncer: ensaios in vitro/in vivo e investigação do mecanismo de ação.
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| Beneficiário: | Giovanna Barbarini Longato |
| Linha de fomento: |
Auxílio à Pesquisa - Regular
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| Processo FAPESP: |
11/50242-5 - Dimensões da vida marinha: padrões e processos de diversificação em cnidários planctônicos e bentônicos
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| Beneficiário: | Antonio Carlos Marques |
| Linha de fomento: |
Auxílio à Pesquisa - Programa BIOTA - Temático
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| Processo FAPESP: |
19/19929-6 - Avaliação de secretases, catepsinas e inflamassomos na doença de alzheimer: novos mecanismos e tratamentos
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| Beneficiário: | Juliana Mozer Sciani |
| Linha de fomento: |
Auxílio à Pesquisa - Regular
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| Processo FAPESP: |
15/50040-4 - Rational approach for searching molecular targets involved in inflammatory events and cell survival
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| Beneficiário: | Ana Marisa Chudzinski-Tavassi |
| Linha de fomento: |
Auxílio à Pesquisa - Programa Centros de Pesquisa em Engenharia
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| Processo FAPESP: |
13/07467-1 - CeTICS - Centro de Toxinas, Imuno-Resposta e Sinalização Celular
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| Beneficiário: | Hugo Aguirre Armelin |
| Linha de fomento: |
Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
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