Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Box Jellyfish (Cnidaria, Cubozoa) Extract Increases Neuron's Connection: A Possible Neuroprotector Effect

Full text
Author(s):
Show less -
Arruda, Gian Lucas M. [1] ; Vigerelli, Hugo [2] ; Bufalo, Michelle C. [3] ; Longato, Giovanna B. [4] ; Veloso, Rodinei V. [1] ; Zambelli, Vanessa O. [3] ; Picolo, Gisele [3] ; Cury, Yara [3] ; Morandini, Andre C. [5, 6] ; Marques, Antonio Carlos [5] ; Sciani, Juliana Mozer [1]
Total Authors: 11
Affiliation:
[1] Univ Sao Francisco, Lab Multidisciplinar Pesquisa, BR-12916900 Braganca Paulista, SP - Brazil
[2] Inst Butantan, Lab Genet, BR-05503900 Sao Paulo - Brazil
[3] Inst Butantan, Lab Dor & Sinalizacao, BR-05503900 Sao Paulo - Brazil
[4] Univ Sao Francisco, Lab Pesquisa Farmacol Mol & Compostos Bioat, BR-12916900 Braganca Paulista, SP - Brazil
[5] Univ Sao Paulo, Inst Biociencias, Dept Zool, BR-05508090 Sao Paulo - Brazil
[6] Univ Sao Paulo, Ctr Biol Marinha, BR-11612109 Sao Sebastiao - Brazil
Total Affiliations: 6
Document type: Journal article
Source: BIOMED RESEARCH INTERNATIONAL; v. 2021, MAR 5 2021.
Web of Science Citations: 0
Abstract

Neurodegenerative diseases are one of the major causes of death worldwide, characterized by neurite atrophy, neuron apoptosis, and synapse loss. No effective treatment has been indicated for such diseases so far, and the search for new drugs is being increased in the last years. Animal venoms' secretion/venom can be an alternative for the discovery of new molecules, which could be the prototype for a new treatment. Here, we present the biochemical characterization and activity of the extract from the box jellyfish Chiropsalmus quadrumanus (Cq) on neurites. The Cq methanolic extract was obtained and incubated to human SH-SY5Y neurons, and neurite parameters were evaluated. The extract was tested in other cell types to check its cytotoxicity and was submitted to biochemical analysis by mass spectrometry in order to check its composition. We could verify that the Cq extract increased neurite outgrowth length and branching junctions, amplifying the contact between SH-SY5Y neurons, without affecting cell body and viability. The extract action was selective for neurons, as it did not cause any effects on other cell types, such as tumor line, nontumor line, and red blood cells. Moreover, mass spectrometry analysis revealed that there are no proteins but several low molecular mass compounds and peptides. Three peptides, characterized as cryptides, and 14 low molecular mass compounds were found to be related to cytoskeleton reorganization, cell membrane expansion, and antioxidant/neuroprotective activity, which act together to increase neuritogenesis. After this evaluation, we conclude that the Cq extract is a promising tool for neuronal connection recovery, an essential condition for the treatment of neurodegenerative diseases. (AU)

FAPESP's process: 15/21007-9 - Recognizing the diversity of jellyfishes (Medusozoa, Rhopaliophora)
Grantee:André Carrara Morandini
Support Opportunities: Regular Research Grants
FAPESP's process: 15/50040-4 - Rational approach for searching molecular targets involved in inflammatory events and cell survival
Grantee:Ana Marisa Chudzinski-Tavassi
Support Opportunities: Research Grants - Research Centers in Engineering Program
FAPESP's process: 16/06137-6 - Bioprospecting for natural compounds and bioactive derivatives with potent anticancer activity: in vitro/in vivo assays and investigation into the mechanism of action.
Grantee:Giovanna Barbarini Longato
Support Opportunities: Regular Research Grants
FAPESP's process: 19/19929-6 - Secretases, cathepsins and inflamasomes evaluation for Alzheimer’s Disease: new pathways and treatments
Grantee:Juliana Mozer Sciani
Support Opportunities: Regular Research Grants
FAPESP's process: 13/07467-1 - CeTICS - Center of Toxins, Immune-Response and Cell Signaling
Grantee:Hugo Aguirre Armelin
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 11/50242-5 - Dimensions of marine life: patterns and process of diversifications in planktonic and benthic cnidarians
Grantee:Antonio Carlos Marques
Support Opportunities: BIOTA-FAPESP Program - Thematic Grants