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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Mast cells contribute to alveolar bone loss in Spontaneously Hypertensive Rats with periodontal disease regulating cytokines production

Texto completo
Autor(es):
Balera Brito, Victor Gustavo [1, 2] ; Patrocinio, Mariana Sousa [1] ; Linjardi Sousa, Maria Carolina [1] ; Alves Barreto, Ayna Emanuelli [1, 2] ; Tfaile Frasnelli, Sabrina Cruz [1] ; Lara, Vanessa Soares [3] ; Santos, Carlos Ferreira [4] ; Penha Oliveira, Sandra Helena [1, 2]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ, UNESP, Sch Dent, Dept Basic Sci, Aracatuba, SP - Brazil
[2] Sao Paulo State Univ, UNESP, Sch Dent, SBFis, Programa Multicentr Posgrad Ciencias Fisiol, Aracatuba, SP - Brazil
[3] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Sao Paulo, SP - Brazil
[4] Univ Sao Paulo, Bauru Sch Dent, Dept Stomatol, Sao Paulo, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 16, n. 3 MAR 4 2021.
Citações Web of Science: 0
Resumo

Mast cells (MCs) play a pivotal role in inflammatory responses and had been studied in inflammatory bone disorders, however, their role in alveolar bone loss induced by periodontal disease (PD) is not yet fully understood. We, therefore, aimed to evaluate the effects of MCs depletion in the PD-induced alveolar bone loss in Wistar (W) and Spontaneously Hypertensive Rats (SHRs). PD was induced by ligating the lower first molars with silk thread one day after the MCs depletion, by the pre-treatment with compound 48/80 for 4 days. After 15 days of PD induction, the hemi-mandibles were surgically collected for qRT-PCR, histological analyses, immunostaining, and ELISA. Systolic blood pressure (SBP) was verified by tail plethysmography to confirm the hypertensive status, and SHR presented SBP >150 mmHg, and previous MC depletion alone or associated with PD did not alter this parameter. SHRs showed a more severe alveolar bone loss compared to W, and MC depletion significantly inhibited this response in both strains, with a more significant response in SHRs. MCs were less abundant in 48/80+PD groups, thus validating the previous MCs depletion in our model. PD increased the number of MC in the gingival tissue of SHR. Cytokine production (TNF-alpha, IL-6, IL-1 beta, and CXCL3) was constitutively higher in SHR and increased further after PD, which was also significantly reduced in the MCs-depleted animals. PD led to an increased expression of Opn, Rankl, Rank, Vtn, Itga5, Itgb5, Trap, and Ctsk in the mandible of W and SHRs, which was reversed in MCs-depleted animals. These results suggest that MCs significantly contributes to the PD-induced alveolar bone resorption, especially in the SHR, which is associated with a more severe PD progression compared to Wistar, partly explained by these cells contribution to the inflammatory status and mediator production, stimulating osteoclast-related response markers, which were reduced after MC depletion in our experimental model. (AU)

Processo FAPESP: 18/23676-3 - Participação dos microRNAs nas alterações ósseas locais induzidas pela doença periodontal e pelo sistema renina-angiotensina local em ratos espontaneamente hipertensos
Beneficiário:Victor Gustavo Balera Brito
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 17/05873-3 - Análise in vitro do papel dos mastócitos e do sistema renina-angiotensina sobre osteoblastos e osteoclastos de animais espontaneamente hipertensos
Beneficiário:Sabrina Cruz Tfaile Frasnelli
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 17/07095-8 - Papel dos mastócitos sobre a diferenciação osteogênica in vitro de células mesenquimais estromais da medula óssea de camundongos
Beneficiário:Maria Carolina Linjardi de Sousa
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 17/02271-2 - O papel do Telmisartan na diferenciação osteoblástica em animais espontaneamente hipertensos
Beneficiário:Mariana Sousa Patrocinio
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 15/03965-2 - Papel do sistema renina-angiotensina em diferentes modelos inflamatórios orais: uma abordagem interdisciplinar experimental e clínica
Beneficiário:Carlos Ferreira dos Santos
Linha de fomento: Auxílio à Pesquisa - Temático