Growth hormone receptor in dopaminergic neurones regulates stress-induced prolactin release in male mice

Autor(es):Mostrar menos -

Wasinski, Frederick ^[1] ; Chaves, Fernanda M. ^[1] ; Pedroso, Joao A. B. ^[1] ; Mansano, Naira S. ^[2] ; Camporez, Joao Paulo ^[3] ; Gusmao, Daniela O. ^[4] ; List, Edward O. ^{[5, 6]} ; Kopchick, John J. ^{[5, 6]} ; Frazao, Renata ^[2] ; Szawka, Raphael E. ^[4] ; Donato, Jr., Jose ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Fisiol & Biofis, Ave Prof Lineu Prestes 1524, BR-05508000 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Inst Ciencias Biomed, Dept Anat, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Fisiol, Ribeirao Preto - Brazil

^[4] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Fisiol & Biofis, Belo Horizonte, MG - Brazil

^[5] Ohio Univ, Edison Biotechnol Inst, Athens, OH 45701 - USA

^[6] Ohio Univ, Heritage Coll Osteopath Med, Athens, OH 45701 - USA

• Tipo de documento: Artigo Científico
• Fonte: Journal of Neuroendocrinology; v. 33, n. 3 MAR 2021.
• Citações Web of Science: 0

Resumo

Arcuate nucleus (ARH) dopaminergic neurones regulate several biological functions, including prolactin secretion and metabolism. These cells are responsive to growth hormone (GH), although it is still unknown whether GH action on ARH dopaminergic neurones is required to regulate different physiological aspects. Mice carrying specific deletion of GH receptor (GHR) in tyrosine hydroxylase (TH)- or dopamine transporter (DAT)-expressing cells were produced. We investigated possible changes in energy balance, glucose homeostasis, fertility, pup survival and restraint stress-induced prolactin release. GHR deletion in DAT- or TH-expressing cells did not cause changes in food intake, energy expenditure, ambulatory activity, nutrient oxidation, glucose tolerance, insulin sensitivity and counter-regulatory response to hypoglycaemia in male and female mice. In addition, GHR deletion in dopaminergic cells caused no gross effects on reproduction and pup survival. However, restraint stress-induced prolactin release was significantly impaired in DAT- and TH-specific GHR knockout male mice, as well as in pegvisomant-treated wild-type males, whereas an intact response was observed in females. Patch clamp recordings were performed in ARH DAT neurones and, in contrast to prolactin, GH did not cause acute changes in the electrical activity of DAT neurones. Furthermore, TH phosphorylation at Ser(40) in ARH neurones and median eminence axonal terminals was not altered in DAT-specific GHR knockout male mice during restraint stress. In conclusion, GH action in dopaminergic neurones is required for stress-induced prolactin release in male mice, suggesting the existence of sex differences in the capacity of GHR signalling to affect prolactin secretion. The mechanism behind this regulation still needs to be identified. (AU)

• Processo FAPESP: 18/04956-5 - Impacto do receptor de estrogênio alpha na Doença Hepática Gordurosa não Alcoólica e metabolismo energético do fígado
• Beneficiário: João Paulo Gabriel Camporez
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 17/02983-2 - Ação do hormônio do crescimento no sistema nervoso: relevância para as funções neurais e na doença
• Beneficiário: Jose Donato Junior
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 20/01318-8 - Sistema nervoso central como um alvo do hormônio do crescimento para a regulação de múltiplas funções biológicas
• Beneficiário: Jose Donato Junior
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 17/21840-8 - Sinalização do hormônio do crescimento no sistema nervoso central como mediador da puberdade e fertilidade
• Beneficiário: Renata Frazão
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/20897-3 - Papel dos neurônios orexina como mediadores dos efeitos centrais induzidos pelo hormônio do crescimento
• Beneficiário: Frederick Wasinski
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/21854-9 - Estudo dos efeitos de citocinas pró- e anti-inflamatórias sobre propriedades biofísicas de neurônios POMC e AgRP do núcleo arqueado do hipotálamo
• Beneficiário: Fernanda Machado Chaves
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/22189-9 - Fatores que alteram a ingestão alimentar modulam a atividade de neurônios Kiss1?
• Beneficiário: Naira da Silva Mansano
• Modalidade de apoio: Bolsas no Brasil - Doutorado