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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Growth hormone receptor in dopaminergic neurones regulates stress-induced prolactin release in male mice

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Author(s):
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Wasinski, Frederick [1] ; Chaves, Fernanda M. [1] ; Pedroso, Joao A. B. [1] ; Mansano, Naira S. [2] ; Camporez, Joao Paulo [3] ; Gusmao, Daniela O. [4] ; List, Edward O. [5, 6] ; Kopchick, John J. [5, 6] ; Frazao, Renata [2] ; Szawka, Raphael E. [4] ; Donato, Jr., Jose [1]
Total Authors: 11
Affiliation:
[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Fisiol & Biofis, Ave Prof Lineu Prestes 1524, BR-05508000 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Inst Ciencias Biomed, Dept Anat, Sao Paulo - Brazil
[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Fisiol, Ribeirao Preto - Brazil
[4] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Fisiol & Biofis, Belo Horizonte, MG - Brazil
[5] Ohio Univ, Edison Biotechnol Inst, Athens, OH 45701 - USA
[6] Ohio Univ, Heritage Coll Osteopath Med, Athens, OH 45701 - USA
Total Affiliations: 6
Document type: Journal article
Source: Journal of Neuroendocrinology; v. 33, n. 3 MAR 2021.
Web of Science Citations: 0
Abstract

Arcuate nucleus (ARH) dopaminergic neurones regulate several biological functions, including prolactin secretion and metabolism. These cells are responsive to growth hormone (GH), although it is still unknown whether GH action on ARH dopaminergic neurones is required to regulate different physiological aspects. Mice carrying specific deletion of GH receptor (GHR) in tyrosine hydroxylase (TH)- or dopamine transporter (DAT)-expressing cells were produced. We investigated possible changes in energy balance, glucose homeostasis, fertility, pup survival and restraint stress-induced prolactin release. GHR deletion in DAT- or TH-expressing cells did not cause changes in food intake, energy expenditure, ambulatory activity, nutrient oxidation, glucose tolerance, insulin sensitivity and counter-regulatory response to hypoglycaemia in male and female mice. In addition, GHR deletion in dopaminergic cells caused no gross effects on reproduction and pup survival. However, restraint stress-induced prolactin release was significantly impaired in DAT- and TH-specific GHR knockout male mice, as well as in pegvisomant-treated wild-type males, whereas an intact response was observed in females. Patch clamp recordings were performed in ARH DAT neurones and, in contrast to prolactin, GH did not cause acute changes in the electrical activity of DAT neurones. Furthermore, TH phosphorylation at Ser(40) in ARH neurones and median eminence axonal terminals was not altered in DAT-specific GHR knockout male mice during restraint stress. In conclusion, GH action in dopaminergic neurones is required for stress-induced prolactin release in male mice, suggesting the existence of sex differences in the capacity of GHR signalling to affect prolactin secretion. The mechanism behind this regulation still needs to be identified. (AU)

FAPESP's process: 18/04956-5 - Impact of the estrogen receptor alpha on Non-Alcoholic Fatty Liver Disease and energetic metabolism of the liver
Grantee:João Paulo Gabriel Camporez
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 17/02983-2 - The role of growth hormone in the brain: relevance for neural functions and in disease
Grantee:Jose Donato Junior
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 20/01318-8 - Central nervous system as a target of growth hormone for the regulation of multiple biological functions
Grantee:Jose Donato Junior
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 17/21840-8 - Growth hormone signaling in the brain as a mediator of puberty and fertility
Grantee:Renata Frazão
Support Opportunities: Regular Research Grants
FAPESP's process: 16/20897-3 - Role of orexin neurons as mediators of the central effects induced by growth hormone
Grantee:Frederick Wasinski
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 17/21854-9 - Investigation of pro- and anti-inflamatory cytokines in biophysical properties of POMC and AgRP neurons of the arcuate nucleus of the hypothalamus
Grantee:Fernanda Machado Chaves
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 17/22189-9 - Does the factors that change food intake modulate the activity of Kiss1 neurons?
Grantee:Naira da Silva Mansano
Support Opportunities: Scholarships in Brazil - Doctorate