Field Deployment of a Mobile Biosafety Laboratory Reveals the Co-Circulation of Dengue Viruses Serotype 1 and Serotype 2 in Louga City, Senegal, 2017

Autor(es):Mostrar menos -

Dieng, Idrissa ^[1] ; Diarra, Maryam ^[2] ; Diagne, Moussa Moise ^[1] ; Faye, Martin ^[1] ; Dior Ndione, Marie Henriette ^[1] ; Ba, Yamar ^[3] ; Diop, Mamadou ^[2] ; Ndiaye, El Hadji ^[3] ; Marinho de Andrade Zanotto, Paolo ^[4] ; Diop, Boly ^[5] ; Ndiaye, Mamadou ^[5] ; Bousso, Abdoulaye ^[5] ; Dia, Ndongo ^[1] ; Diallo, Mawlouth ^[3] ; Barry, Aliou ^[4] ; Fall, Gamou ^[1] ; Loucoubar, Cheikh ^[2] ; Sall, Amadou Alpha ^[1] ; Faye, Ousmane ^[1] ; Faye, Oumar ^[1]

Afiliação do(s) autor(es)

^[1] Inst Pasteur, Dept Virol, Arboviruses & Haemorrhag Fever Viruses Unit, Dakar 220 - Senegal

^[2] Inst Pasteur, Epidemiol Clin Res & Data Sci Unit, Dakar 220 - Senegal

^[3] Inst Pasteur, Med Entomol Unit, 36 Ave, Dakar - Senegal

^[4] Univ SP, Inst Biomed Sci, Dept Microbiol, Lab Mol Evolut & Bioinformat, Sao Paulo, SP - Brazil

^[5] Minist Hlth, Prevent Dept, Dakar - Senegal

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF TROPICAL MEDICINE; v. 2021, APR 1 2021.
• Citações Web of Science: 2

Resumo

Dengue virus (DENV) is the most prevalent arboviral threat worldwide. This virus belonging to genus Flavivirus, Flaviviridae family, is responsible for a wide spectrum of clinical manifestations, ranging from asymptomatic or mild febrile illness (dengue fever) to life-threatening infections (severe dengue). Many sporadic cases and outbreaks have occurred in Senegal since 1970. Nevertheless, this article describes a field investigation of suspected dengue cases, between 05 September 2017 and 17 December 2017 made possible by the deployment of a Mobile Biosafety Laboratory (MBS-Lab). Overall, 960 human sera were collected and tested in the field for the presence of viral RNA by real-time RT-PCR. Serotyping, sequencing of complete E gene, and phylogenetic analysis were also performed. Out of 960 suspected cases, 131 were confirmed dengue cases. The majority of confirmed cases were from Louga community. Serotyping revealed two serotypes, Dengue 1 (100/104; 96, 15%) and Dengue 2 (04/104; 3, 84%). Phylogenetic analysis of the sequences obtained indicated that the Dengue 1 strain was closely related to strains isolated, respectively, in Singapore (Asia) in 2013 (KX380803.1) outbreak and it cocirculated with a Dengue 2 strain closely related to strains from a Burkina Faso dengue outbreak in 2016 (KY62776.1). Our results showed the co-circulation of two dengue virus serotypes during a single outbreak in a short time period. This co-circulation highlighted the need to improve surveillance in order to prevent future potential severe dengue cases through antibody-dependent enhancement (ADE). Interestingly, it also proved the reliability and usefulness of the MBS-Lab for expedient outbreak response at the point of need, which allows early cases management. (AU)

• Processo FAPESP: 17/23281-6 - Utilização de anticorpos que neutralizam a infecção após a adesão à célula para o desenvolvimento de estratégias imunoterápicas inovadoras exemplificadas pela destruição seletiva de células humanas infectadas pelo vírus Zika.
• Beneficiário: Paolo Marinho de Andrade Zanotto
• Modalidade de apoio: Auxílio à Pesquisa - Regular