A novel acrylic resin palatal device contaminated with Candida albicans biofilm for denture stomatitis induction in Wistar rats

Autor(es)

Moraes, Gustavo Simao ^[1] ; Albach, Thais ^[1] ; Ramos, Isabella Esser ^[1] ; Kopacheski, Mariana Gomes ^[1] ; Cachoeira, Victoria Schlumberger ^[1] ; Campos Sugio, Carolina Yoshi ^[2] ; Galvao Arrais, Cesar Augusto ^[1] ; Neppelenbroek, Karin Hermana ^[2] ; Urban, Vanessa Migliorini ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Ponta Grossa, Dept Odontol, Ponta Grossa, Parana - Brazil

^[2] Univ Sao Paulo, Fac Odontol Bauru, Al Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Applied Oral Science; v. 29, 2021.
• Citações Web of Science: 0

Resumo

Abstract Denture stomatitis is the most frequent oral lesion in removable prosthesis wearers, with high recurrence rates and a complex treatment. Objective This study describes a protocol to obtain and to contaminate a palatal device with Candida albicans biofilm that could be used for an animal model of denture stomatitis. Methodology Acrylic resin devices (N=41) were obtained from impressions of the palates of Wistar rats with individual trays and polyether. The efficacy of microwave irradiation (MW), ultraviolet light (UV), or ultrasonic bath (US) was assessed by colony viability and spectrophotometric analyses (n=5) in order to select the most appropriate method for sterilizing the devices. Then, different devices (n=5) were contaminated with C. albicans and evaluated by CFU/mL determination, scanning electron microscopy, and laser confocal microscopy. Device stabilization was assessed with either autopolymerizing acrylic resins or a self-adhesive resin cement (n=2). The spectrophotometric data were analyzed by one-way ANOVA followed by the Tukey’s HSD post-hoc test (α=0.05). Results MW was the only method capable of sterilizing the devices, and the contamination protocol developed a mature and viable C. albicans biofilm (~1.2 x 106 CFU/mL). The self-adhesive resin cement was the best stabilization material. Conclusions This acrylic resin palatal device was designed to be similar to the clinical situation of contaminated prostheses, with easy manufacturing and handling, effective stabilization, and satisfactory contamination. Thus, the acrylic device can be a valuable tool in the development of denture stomatitis in rats. (AU)

• Processo FAPESP: 17/22996-1 - Resposta tecidual em modelo animal de condicionador de tecido contendo complexo de inclusão antifúngico: ²-ciclodextrina para tratamento de estomatite protética
• Beneficiário: Carolina Yoshi Campos Sugio
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 17/07314-1 - Avaliação clínica da adição de antifúngicos a reembasador resiliente temporário no tratamento da estomatite protética
• Beneficiário: Karin Hermana Neppelenbroek
• Modalidade de apoio: Auxílio à Pesquisa - Regular