The Glypican proteoglycans show intrinsic interactions with Wnt-3a in human prostate cancer cells that are not always associated with cascade activation

Autor(es)

de Moraes, Gabrielle Ferrante Alves ^[1] ; Listik, Eduardo ^[1] ; Justo, Giselle Zenker ^{[1, 2]} ; Vicente, Carolina Meloni ^[1] ; Toma, Leny ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Bioquim, Campus Sao Paulo, Rua Tres Maio, POB 04044-020, BR-100 Sao Paulo, SP - Brazil

^[2] Univ Fed Sao Paulo, Dept Ciencias Biol, Campus Diadema, Rua Tres Maio, POB 04044-020, BR-100 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BMC MOLECULAR AND CELL BIOLOGY; v. 22, n. 1 MAY 4 2021.
• Citações Web of Science: 0

Resumo

Background Prostate cancer occurs through multiple steps until advanced metastasis. Signaling pathways studies can result in the identification of targets to interrupt cancer progression. Glypicans are cell surface proteoglycans linked to the membrane through glycosylphosphatidylinositol. Their interaction with specific ligands has been reported to trigger diverse signaling, including Wnt. In this study, prostate cancer cell lines PC-3, DU-145, and LNCaP were compared to normal prostate RWPE-1 cell line to investigate glypican family members and the activation of the Wnt signaling pathway. Results Glypican-1 (GPC1) was highly expressed in all the examined cell lines, except for LNCaP, which expressed glypican-5 (GPC5). The subcellular localization of GPC1 was detected on the cell surface of RWPE-1, PC-3, and DU-145 cell lines, while GPC5 suggested cytoplasm localization in LNCaP cells. Besides glypican, flow cytometry analysis in these prostate cell lines confirmed the expression of Wnt-3a and unphosphorylated beta-catenin. The co-immunoprecipitation assay revealed increased levels of binding between Wnt-3a and glypicans in cancer cells, suggesting a relationship between these proteoglycans in this pathway. A marked increase in nuclear beta-catenin was observed in tumor cells. However, only PC-3 cells demonstrated activation of canonical Wnt signaling, according to the TOPFLASH assay. Conclusions GPC1 was the majorly expressed gene in all the studied cell lines, except for LNCaP, which expressed GPC5. We assessed by co-immunoprecipitation that these GPCs could interact with Wnt-3a. However, even though nuclear beta-catenin was found increased in the prostate cancer cells (i.e., PC-3, DU-145 and LNCaP), activation of Wnt pathway was only found in PC-3 cells. In these PC-3 cells, GPC1 and Wnt-3a revealed high levels of colocalization, as assessed by confocal microscopy studies. This suggests a localization at the cellular surface, where Frizzled receptor is required for downstream activation. The interaction of Wnt-3a with GPCs in DU-145 and LNCaP cells, which occurs in absence of Wnt signaling activation, requires further studies. Once non-TCF-LEF proteins can also bind beta-catenin, another signaling pathway may be involved in these cells with regulatory function. (AU)

• Processo FAPESP: 16/18066-6 - O papel dos microdomínios 6-O-sulfatados de proteoglicanos na biologia tumoral e tecidual.
• Beneficiário: Leny Toma
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/50024-0 - Papel dos proteoglicanos na biologia do cancer coloretal e de prostata humanos:um estudo in vitro do seu microambiente tumoral.
• Beneficiário: Leny Toma
• Modalidade de apoio: Auxílio à Pesquisa - Regular