Frequency and Diversity of Hybrid Escherichia coli Strains Isolated from Urinary Tract Infections

Autor(es)

Nascimento, Jullia A. S. ^[1] ; Santos, Fernanda F. ^{[1, 2]} ; Valiatti, Tiago B. ^{[1, 2]} ; Santos-Neto, Jose F. ^[1] ; Santos, Ana Carolina M. ^[1] ; Cayo, Rodrigo ^{[2, 3]} ; Gales, Ana C. ^[2] ; Gomes, Tania A. T. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo UNIFESP, Escola Paulista Med EPM, Dept Microbiol Imunol & Parasitol DMIP, Lab Expt Patogenicidade Enterobacterias LEPE, Disc, BR-04023062 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo UNIFESP, Dept Med, Escola Paulista Med EPM, Lab Alerta, Disciplina Infectol, BR-04039032 Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo UNIFESP, Inst Ciencias Ambientais Quim & Farmaceut ICAQF, Dept Ciencias Biol DCB, Lab Imunol & Microbiol LIB, Setor Biol Mol Microbi, BR-09972270 Diadema - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MICROORGANISMS; v. 9, n. 4 APR 2021.
• Citações Web of Science: 0

Resumo

(1) Background: Hybrid uropathogenic Escherichia coli (UPEC) strains carry virulence markers of the diarrheagenic E. coli (DEC) pathotypes, which may increase their virulence potential. This study analyzed the frequency and virulence potential of hybrid strains among 452 UPEC strains. (2) Methods: Strains were tested for the DEC virulence diagnostic genes' presence by polymerase chain reaction (PCR). Those carrying at least one gene were classified as hybrid and further tested for 10 UPEC and extraintestinal pathogenic E. coli (ExPEC) virulence genes and phylogenetic classification. Also, their ability to produce hemolysis, adhere to HeLa and renal HEK 293T cells, form a biofilm, and antimicrobial susceptibility were evaluated. (3) Results: Nine (2%) hybrid strains were detected; seven of them carried aggR and two, eae, and were classified as UPEC/EAEC (enteroaggregative E. coli) and UPEC/aEPEC (atypical enteropathogenic E. coli), respectively. They belonged to phylogroups A (five strains), B1 (three), and D (one), and adhered to both cell lineages tested. Only the UPEC/EAEC strains were hemolytic (five strains) and produced biofilm. One UPEC/aEPEC strain was resistant to third-generation cephalosporins and carried bla(CTX-M-15). (4) Conclusions: Our findings contribute to understanding the occurrence and pathogenicity of hybrid UPEC strains, which may cause more severe infections. (AU)

• Processo FAPESP: 17/21947-7 - Análise do genoma e do potencial de virulência de uma cepa de Escherichia coli uropatogênica (UPEC) portadora de marcadores de E. coli diarreiogênica (DEC)
• Beneficiário: Tiago Barcelos Valiatti
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 18/17353-7 - ANÁLISE COMPARATIVA DE CARACTERÍSTICAS DE VIRULÊNCIA DE Escherichia coli DIARREIOGÊNICA EM CEPAS DE E. coli ISOLADAS DE PACIENTES COM DIFERENTES TIPOS DE INFECÇÃO URINÁRIA
• Beneficiário: Tânia Aparecida Tardelli Gomes do Amaral
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/14553-8 - Análise comparativa de características de virulência de Escherichia coli diarreiogênica em cepas de E. coli isoladas de pacientes com diferentes tipos de infecção urinária
• Beneficiário: Júllia Assis da Silva Nascimento
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 19/21685-8 - Estudo de marcadores de virulência presentes em cepas de Escherichia coli isoladas da microbiota intestinal de pacientes com infecção urinária
• Beneficiário: José Francisco Santos Neto
• Modalidade de apoio: Bolsas no Brasil - Mestrado