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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Frequency and Diversity of Hybrid Escherichia coli Strains Isolated from Urinary Tract Infections

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Author(s):
Nascimento, Jullia A. S. [1] ; Santos, Fernanda F. [1, 2] ; Valiatti, Tiago B. [1, 2] ; Santos-Neto, Jose F. [1] ; Santos, Ana Carolina M. [1] ; Cayo, Rodrigo [2, 3] ; Gales, Ana C. [2] ; Gomes, Tania A. T. [1]
Total Authors: 8
Affiliation:
[1] Univ Fed Sao Paulo UNIFESP, Escola Paulista Med EPM, Dept Microbiol Imunol & Parasitol DMIP, Lab Expt Patogenicidade Enterobacterias LEPE, Disc, BR-04023062 Sao Paulo - Brazil
[2] Univ Fed Sao Paulo UNIFESP, Dept Med, Escola Paulista Med EPM, Lab Alerta, Disciplina Infectol, BR-04039032 Sao Paulo - Brazil
[3] Univ Fed Sao Paulo UNIFESP, Inst Ciencias Ambientais Quim & Farmaceut ICAQF, Dept Ciencias Biol DCB, Lab Imunol & Microbiol LIB, Setor Biol Mol Microbi, BR-09972270 Diadema - Brazil
Total Affiliations: 3
Document type: Journal article
Source: MICROORGANISMS; v. 9, n. 4 APR 2021.
Web of Science Citations: 0
Abstract

(1) Background: Hybrid uropathogenic Escherichia coli (UPEC) strains carry virulence markers of the diarrheagenic E. coli (DEC) pathotypes, which may increase their virulence potential. This study analyzed the frequency and virulence potential of hybrid strains among 452 UPEC strains. (2) Methods: Strains were tested for the DEC virulence diagnostic genes' presence by polymerase chain reaction (PCR). Those carrying at least one gene were classified as hybrid and further tested for 10 UPEC and extraintestinal pathogenic E. coli (ExPEC) virulence genes and phylogenetic classification. Also, their ability to produce hemolysis, adhere to HeLa and renal HEK 293T cells, form a biofilm, and antimicrobial susceptibility were evaluated. (3) Results: Nine (2%) hybrid strains were detected; seven of them carried aggR and two, eae, and were classified as UPEC/EAEC (enteroaggregative E. coli) and UPEC/aEPEC (atypical enteropathogenic E. coli), respectively. They belonged to phylogroups A (five strains), B1 (three), and D (one), and adhered to both cell lineages tested. Only the UPEC/EAEC strains were hemolytic (five strains) and produced biofilm. One UPEC/aEPEC strain was resistant to third-generation cephalosporins and carried bla(CTX-M-15). (4) Conclusions: Our findings contribute to understanding the occurrence and pathogenicity of hybrid UPEC strains, which may cause more severe infections. (AU)

FAPESP's process: 17/21947-7 - Analysis of the genome and virulence potential of a uropathogenic Escherichia coli (UPEC) strain carrying diarrheogenic E. coli (DEC) markers
Grantee:Tiago Barcelos Valiatti
Support type: Scholarships in Brazil - Master
FAPESP's process: 18/17353-7 - COMPARATIVE ANALYSIS OF VIRULENCE CHARACTERISTICS OF DIARRHEIOGENIC Escherichia coli IN E. coli STRAINS ISOLATED FROM PATIENTS WITH DIFFERENT TYPES OF URINARY INFECTION
Grantee:Tânia Aparecida Tardelli Gomes do Amaral
Support type: Regular Research Grants
FAPESP's process: 19/14553-8 - Comparative analysis of virulence characteristics of Diarrheagenic Escherichia coli in strains of e. coli isolated from patients with different types of urinary infection
Grantee:Júllia Assis da Silva Nascimento
Support type: Scholarships in Brazil - Master
FAPESP's process: 19/21685-8 - Studying the presence of virulence markers in Escherichia coli strains isolated from the gut microbiota of patients with urinary tract infection
Grantee:José Francisco Santos Neto
Support type: Scholarships in Brazil - Master