Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile(21))-Ha antimicrobial peptide application as a feed additive

Autor(es):Mostrar menos -

Roque-Borda, Cesar Augusto ^[1] ; Leite Silva, Hanyeny Raiely ^[1] ; Crusca Junior, Edson ^[2] ; Serafim, Jessica Aparecida ^[3] ; Meneguin, Andreia Bagliotti ^[4] ; Chorilli, Marlus ^[4] ; Macedo, Wagner Costa ^[5] ; Teixeira, Silvio Rainho ^[5] ; Lopes Guastalli, Elisabete Aparecida ^[6] ; Soares, Nilce Maria ^[6] ; Blair, Jessica M. A. ^[7] ; Pikramenou, Zoe ^[8] ; Vicente, Eduardo Festozo ^[3]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Sch Agr & Vet Sci, BR-14884900 Jaboticabal, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Inst Chem, BR-14800900 Araraquara, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Sch Sci & Engn, BR-17602496 Tupa, SP - Brazil

^[4] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, BR-14801902 Araraquara, SP - Brazil

^[5] Sao Paulo State Univ Unesp, Sch Technol & Sci, BR-19060900 Presidente Prodente, SP - Brazil

^[6] Biol Inst, Poultry Hlth Specialized Lab, BR-17690000 Bastos, SP - Brazil

^[7] Univ Birmingham, Inst Microbiol & Infect, Birmingham B15 2TT, W Midlands - England

^[8] Univ Birmingham, Sch Chem, Birmingham B15 2TT, W Midlands - England

• Tipo de documento: Artigo Científico
• Fonte: International Journal of Biological Macromolecules; v. 183, p. 1236-1247, JUL 31 2021.
• Citações Web of Science: 4

Resumo

Microencapsulation is a potential biotechnological tool, which can overcome antimicrobial peptides (AMP) instabilities and reduce toxic side effects. Thus, this study evaluates the antibacterial activities of the Ctx(Ile(21))-Ha AMP against multidrug-resistant (MDR) and non-resistant bacteria and develop and characterize peptide loaded microparticles coated with the enteric polymers hydroxypropylmethylcellulose acetate succinate (HPMCAS) and hydroxypropylmethylcellulose phthalate (HPMCP). Ctx(Ile(21))-Ha was obtained by solid phase peptide synthesis (SPPS) method, purified and characterized by HPLC and Mass Spectrometry. The peptide exhibited potent antibiotic activities against Salmonella enteritidis, Salmonella typhimurium, Pseudomonas aeruginosa (MDR), Acinetobacter baumannii (MDR), and Staphylococcus aureus (MDR). Ctx(Ile(21))-Ha microencapsulation was performed by ionic gelation with high efficiency, maintaining the physical-chemical stability. Ctx(Ile(21))-Ha coated-microparticles were characterized by DSC, TGA, FTIR-Raman, XRD and SEM. Hemolytic activity assay demonstrated that hemolysis was decreased up to 95% compared to single molecule. In addition, in vitro release control profile simulating different portions of gastrointestinal tract was performed and showed the microcapsules' ability to protect the peptide and release it in the intestine, aiming pathogen's location, mainly by Salmonella sp. Therefore, use of microencapsulated Ctx(Ile(21))-Ha can be allowed as an antimicrobial controller in monogastric animal production as an oral feed additive (antimicrobial controller), being a valuable option for molecules with low therapeutic indexes or high hemolytic rates. (C) 2021 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 18/25707-3 - Aplicação do peptídeo antimicrobiano Ctx(Ile21)-Ha e seus conjugados em diferentes formulações como aditivo nutricional em pintainhas de postura
• Beneficiário: Cesar Augusto Roque Borda
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 16/00446-7 - Aplicação de peptídeos antimicrobianos como novos aditivos na nutrição animal
• Beneficiário: Eduardo Festozo Vicente
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 18/25735-7 - Síntese e aplicação do peptídeo antimicrobiano Ctx(Ile21)-Ha e seus conjugados em diferentes formulações como aditivo nutricional em suínos
• Beneficiário: Hanyeny Raiely Leite Silva
• Modalidade de apoio: Bolsas no Brasil - Mestrado