| Full text | |
| Author(s): Show less - |
Roque-Borda, Cesar Augusto
[1]
;
Leite Silva, Hanyeny Raiely
[1]
;
Crusca Junior, Edson
[2]
;
Serafim, Jessica Aparecida
[3]
;
Meneguin, Andreia Bagliotti
[4]
;
Chorilli, Marlus
[4]
;
Macedo, Wagner Costa
[5]
;
Teixeira, Silvio Rainho
[5]
;
Lopes Guastalli, Elisabete Aparecida
[6]
;
Soares, Nilce Maria
[6]
;
Blair, Jessica M. A.
[7]
;
Pikramenou, Zoe
[8]
;
Vicente, Eduardo Festozo
[3]
Total Authors: 13
|
| Affiliation: | [1] Sao Paulo State Univ UNESP, Sch Agr & Vet Sci, BR-14884900 Jaboticabal, SP - Brazil
[2] Sao Paulo State Univ UNESP, Inst Chem, BR-14800900 Araraquara, SP - Brazil
[3] Sao Paulo State Univ UNESP, Sch Sci & Engn, BR-17602496 Tupa, SP - Brazil
[4] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, BR-14801902 Araraquara, SP - Brazil
[5] Sao Paulo State Univ Unesp, Sch Technol & Sci, BR-19060900 Presidente Prodente, SP - Brazil
[6] Biol Inst, Poultry Hlth Specialized Lab, BR-17690000 Bastos, SP - Brazil
[7] Univ Birmingham, Inst Microbiol & Infect, Birmingham B15 2TT, W Midlands - England
[8] Univ Birmingham, Sch Chem, Birmingham B15 2TT, W Midlands - England
Total Affiliations: 8
|
| Document type: | Journal article |
| Source: | International Journal of Biological Macromolecules; v. 183, p. 1236-1247, JUL 31 2021. |
| Web of Science Citations: | 4 |
| Abstract | |
Microencapsulation is a potential biotechnological tool, which can overcome antimicrobial peptides (AMP) instabilities and reduce toxic side effects. Thus, this study evaluates the antibacterial activities of the Ctx(Ile(21))-Ha AMP against multidrug-resistant (MDR) and non-resistant bacteria and develop and characterize peptide loaded microparticles coated with the enteric polymers hydroxypropylmethylcellulose acetate succinate (HPMCAS) and hydroxypropylmethylcellulose phthalate (HPMCP). Ctx(Ile(21))-Ha was obtained by solid phase peptide synthesis (SPPS) method, purified and characterized by HPLC and Mass Spectrometry. The peptide exhibited potent antibiotic activities against Salmonella enteritidis, Salmonella typhimurium, Pseudomonas aeruginosa (MDR), Acinetobacter baumannii (MDR), and Staphylococcus aureus (MDR). Ctx(Ile(21))-Ha microencapsulation was performed by ionic gelation with high efficiency, maintaining the physical-chemical stability. Ctx(Ile(21))-Ha coated-microparticles were characterized by DSC, TGA, FTIR-Raman, XRD and SEM. Hemolytic activity assay demonstrated that hemolysis was decreased up to 95% compared to single molecule. In addition, in vitro release control profile simulating different portions of gastrointestinal tract was performed and showed the microcapsules' ability to protect the peptide and release it in the intestine, aiming pathogen's location, mainly by Salmonella sp. Therefore, use of microencapsulated Ctx(Ile(21))-Ha can be allowed as an antimicrobial controller in monogastric animal production as an oral feed additive (antimicrobial controller), being a valuable option for molecules with low therapeutic indexes or high hemolytic rates. (C) 2021 Elsevier B.V. All rights reserved. (AU) | |
| FAPESP's process: | 18/25707-3 - Application of the antimicrobial peptide Ctx(Ile21)-Ha and its conjugates in different formulations as a nutritional additive in laying hens |
| Grantee: | Cesar Augusto Roque Borda |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 16/00446-7 - Application of antimicrobial peptides as new additives in animal nutrition |
| Grantee: | Eduardo Festozo Vicente |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 18/25735-7 - Synthesis and application of the antimicrobial peptide Ctx(Ile21)-Ha and its conjugates in different formulations as a nutritional additive in swines |
| Grantee: | Hanyeny Raiely Leite Silva |
| Support Opportunities: | Scholarships in Brazil - Master |