Occurrence of multidrug-resistant Enterococcus faecium isolated from environmental samples

Autor(es)

dos Santos, L. D. R. ^[1] ; Furlan, J. P. R. ^[1] ; Gallo, I. F. L. ^[1] ; Ramos, M. S. ^[1] ; Savazzi, E. A. ^[2] ; Stehling, E. G. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Anal Clin Toxicol & Bromatol, Fac Ciencias Farmaceut Ribeirao Preto, Sao Paulo - Brazil

^[2] Companhia Ambiental Estado Sao Paulo CETESB, Ribeirao Preto - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Letters in Applied Microbiology; v. 73, n. 2, p. 237-246, AUG 2021.
• Citações Web of Science: 0

Resumo

Enterococcus species are present in the microbiota of humans and animals and have also been described in the environment. Among the species, Enterococcus faecium is one of the main pathogens associated with nosocomial infections worldwide. Enterococcus faecium isolates resistant to different classes of antimicrobials have been increasingly reported, including multidrug-resistant (MDR) isolates in environmental sources, which is worrying. Therefore, this study aimed to characterize E. faecium isolates obtained from soil and water samples regarding antimicrobial resistance and virulence determinants. A total 40 E. faecium isolates were recovered from 171 environmental samples. All isolates were classified as MDR, highlighting the resistance to the fluoroquinolones class, linezolid and vancomycin. Furthermore, high-level aminoglycoside resistance and high-level ciprofloxacin resistance were detected in some isolates. Several clinically relevant antimicrobial resistance genes were found, including vanC1, ermB, ermC, mefAE, tetM, tetL, ant(6 `)-Ia, ant(4 `)-Ia, aph(3 `)-IIIa and aac(6 `)-Ie-aph(2 `')-Ia. Three virulence genes were detected among the MDR E. faecium isolates, such as esp, gelE and ace. The results of this study contribute to a better understanding of MDR E. faecium isolates carrying antimicrobial resistance and virulence genes in environmental sources and report for the first time in the world the presence of vanC1-producing E. faecium isolated from soil. (AU)

• Processo FAPESP: 18/01890-3 - Estudo do perfil de resistência, virulência e epidemiológico de Escherichia coli isoladas do meio ambiente
• Beneficiário: João Pedro Rueda Furlan
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 18/19539-0 - Caracterização molecular de isolados de Escherichia coli provenientes do meio ambiente
• Beneficiário: Eliana Guedes Stehling
• Modalidade de apoio: Auxílio à Pesquisa - Regular