Differentiation of Porcine Induced Pluripotent Stem Cells (piPSCs) into Neural Progenitor Cells (NPCs)

Autor(es)

Machado, Lucas Simoes ^[1] ; Recchia, Kaiana ^{[1, 2]} ; Godoy Pieri, Naira Caroline ^[2] ; Botigelli, Ramon Cesar ^{[2, 3]} ; Guimaraes de Castro, Raquel Vasconcelos ^[2] ; Cruz, Jessica Brunhara ^[1] ; de Figueiredo Pessoa, Lais Vicari ^[2] ; Bressan, Fabiana Fernandes ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Surg, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, Sao Paulo - Brazil

^[3] Sao Paulo State Univ, Inst Biosci, Dept Pharmacol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOVE-JOURNAL OF VISUALIZED EXPERIMENTS; n. 172 JUN 2021.
• Citações Web of Science: 0

Resumo

iPSC-derived neurons are attractive in vitro models to study neurogenesis and early phenotypic changes in mental illness, mainly when most animal models used in preclinical research, such as rodents, are not able to meet the criteria to translate the findings to the clinic. Non-human primates, canines, and porcine are considered more adequate models for biomedical research and drug development purposes, mainly due to their physiological, genetic, and anatomical similarities to humans. The swine model has gained particular interest in translational neuroscience, enabling safety and allotransplantation testing. Herein the generation of porcine iPSCs is described along with its further differentiation into neural progenitor cells (NPCs). The generated cells expressed NPC markers Nestin and GFAP, confirmed by RT-qPCR, and were positive for Nestin, b-Tubulin III, and Vimentin by immunofluorescence. These results show the evidence for the generation of NPC-like cells after in vitro induction with chemical inhibitors from a large animal model, an interesting and adequate model for regenerative and translational medicine research. (AU)

• Processo FAPESP: 17/13973-8 - Geração e caracterização funcional de células neurais derivadas de iPSCs de pacientes com doenças neurodegenerativas
• Beneficiário: Lucas Simões Machado
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Mestrado
• Processo FAPESP: 15/26818-5 - Investigação de mecanismos celulares e moleculares da aquisição da toti- e pluripotência induzida in vitro - modelo translacional
• Beneficiário: Fabiana Fernandes Bressan
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 17/02159-8 - Indução da pluripotência celular e diferenciação in vitro no modelo suíno como modelo translacional
• Beneficiário: Lucas Simões Machado
• Modalidade de apoio: Bolsas no Brasil - Mestrado