Novel Method for the Detection of SARS-CoV-2 Based on Graphene-Impedimetric Immunosenso

Autor(es)

Zaccariotto, Gabriel C. ^[1] ; Silva, Martin K. L. ^[1] ; Rocha, Giovanna S. ^[1] ; Cesarino, Ivana ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Sch Agr, Dept Bioproc & Biotechnol, BR-18610034 Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MATERIALS; v. 14, n. 15 AUG 2021.
• Citações Web of Science: 2

Resumo

Due to the SARS-CoV-2 pandemic, there has been an increase in the search for affordable healthcare devices for mass testing and rapid diagnosis. In this context, this work described a new methodology for SARS-CoV-2 detection based on an impedimetric immunosensor developed using the advantageous immobilization of antibodies in the reduced graphene oxide (rGO). The rGO was obtained by chemical synthesis from the commercial graphene oxide (GO), and the materials were morphologically, electrochemically and visually characterized. The cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques were used to evaluate the fabrication steps of the immunosensor. The electrochemical immunoassay was considered for SARS-CoV-2 spike protein RBD detection using a impedimetric immunosensor and redox couple ({[}(Fe(CN)(6))](3-/4-)) as a probe. The immunosensor was effectively developed and applied in the detection of SARS-CoV-2 spike protein RBD in saliva samples. (AU)

• Processo FAPESP: 17/24274-3 - Desenvolvimento de biossensor de glicose como ferramenta analítica visando a otimização da produção de etanol de segunda geração a partir de resíduos do abacaxizeiro
• Beneficiário: Ivana Cesarino
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular