Biocomposites based on SBA-15 and papain: Characterization, enzymatic activity and cytotoxicity evaluation

Autor(es)

Losito, Danilo W. ^[1] ; Lopes, Patricia S. ^[2] ; Ueoka, Andreza R. ^[2] ; Fantini, Marcia C. A. ^[3] ; Oseliero Filho, Pedro Leonidas ^[3] ; Andreo-Filho, Newton ^[4] ; Martins, Tereza S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Lab Mat Hibridos, Diadema, SP - Brazil

^[2] Univ Fed Sao Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Lab Imunol Celular & Bioquim Fungos & Protozoario, Diadema, SP - Brazil

^[3] Univ Sao Paulo, Inst Fis, Sao Paulo, SP - Brazil

^[4] Univ Fed Sao Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Lab Farmacotecn & Cosmetol, Diadema, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Microporous and Mesoporous Materials; v. 325, OCT 2021.
• Citações Web of Science: 0

Resumo

Papain has bacteriostatic and anti-inflammatory properties and has been shown to be effective in the healing process of wounds, injuries, and ulcers by breaking down fibrinous material into necrotic tissue. However, papain is unstable and easily denatures or deactivates preventing their broad industrial application. In this work, papain was incorporated into SBA-15 ordered mesoporous silica, with the aim of improving its stability, as well as its encapsulation yield and influence of the reaction time on its stability and enzymatic activity. By small-angle X-ray scattering it was possible to verify that the papain is a globular and slightly elongated protein, likely dimeric, with a maximum size of -6.0 nm, which favors its inclusion in the SBA-15 mesopores (-10 nm). The biocomposites (SBA-15:papain) were characterized by several physical-chemical techniques. The results indicated that the papain was incorporated into the SBA-15 mesopores. The biocomposites exhibited higher thermal degradation temperature than pure papain and did not show cytotoxic potential. The papain enzymatic activity was maintained, with the best performance for the samples prepared with 30 wt% of papain. The results also showed that the 3D cell culture system is better suited for biocomposites that present a cell disruption mechanism. (AU)

• Processo FAPESP: 19/12301-1 - Síntese de sílicas mesoporosas ordenadas com diferentes estruturas e morfologias
• Beneficiário: Pedro Leonidas Oseliero Filho
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/26618-1 - Avaliação in vitro de substâncias lipogênicas e anti-adipogênicas utilizando modelo tridimensional de adipócitos
• Beneficiário: Patricia Santos Lopes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/17844-8 - Sílica nanoestruturada como veículo protetor de vacinas e biomoléculas
• Beneficiário: Osvaldo Augusto Brazil Esteves Sant'Anna
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 16/22916-5 - Desenvolvimento de minicomprimidos veiculando papaína livre e complexada com ciclodextrina e alginato de sódio para liberação no intestino delgado e cólon
• Beneficiário: Newton Andréo Filho
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 20/13204-7 - O papel da morfologia da sílica mesoporosa na incorporação das anatoxinas de difteria e tétano: potenciais adjuvantes para vacinas orais
• Beneficiário: Pedro Leonidas Oseliero Filho
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 19/08582-5 - EMU concedido no processo 17/17844-8:Analisador térmico - TG/DTA modelo sta 2500 TG/DTA TEMP. ambiente a 1100 °c
• Beneficiário: Tereza da Silva Martins
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários