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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Biocomposites based on SBA-15 and papain: Characterization, enzymatic activity and cytotoxicity evaluation

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Losito, Danilo W. [1] ; Lopes, Patricia S. [2] ; Ueoka, Andreza R. [2] ; Fantini, Marcia C. A. [3] ; Oseliero Filho, Pedro Leonidas [3] ; Andreo-Filho, Newton [4] ; Martins, Tereza S. [1]
Total Authors: 7
[1] Univ Fed Sao Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Lab Mat Hibridos, Diadema, SP - Brazil
[2] Univ Fed Sao Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Lab Imunol Celular & Bioquim Fungos & Protozoario, Diadema, SP - Brazil
[3] Univ Sao Paulo, Inst Fis, Sao Paulo, SP - Brazil
[4] Univ Fed Sao Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Lab Farmacotecn & Cosmetol, Diadema, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Microporous and Mesoporous Materials; v. 325, OCT 2021.
Web of Science Citations: 0

Papain has bacteriostatic and anti-inflammatory properties and has been shown to be effective in the healing process of wounds, injuries, and ulcers by breaking down fibrinous material into necrotic tissue. However, papain is unstable and easily denatures or deactivates preventing their broad industrial application. In this work, papain was incorporated into SBA-15 ordered mesoporous silica, with the aim of improving its stability, as well as its encapsulation yield and influence of the reaction time on its stability and enzymatic activity. By small-angle X-ray scattering it was possible to verify that the papain is a globular and slightly elongated protein, likely dimeric, with a maximum size of -6.0 nm, which favors its inclusion in the SBA-15 mesopores (-10 nm). The biocomposites (SBA-15:papain) were characterized by several physical-chemical techniques. The results indicated that the papain was incorporated into the SBA-15 mesopores. The biocomposites exhibited higher thermal degradation temperature than pure papain and did not show cytotoxic potential. The papain enzymatic activity was maintained, with the best performance for the samples prepared with 30 wt% of papain. The results also showed that the 3D cell culture system is better suited for biocomposites that present a cell disruption mechanism. (AU)

FAPESP's process: 19/12301-1 - Synthesis of ordered mesoporous silica with different structures and morphologies
Grantee:Pedro Leonidas Oseliero Filho
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 17/26618-1 - In vitro evaluation of lipogenics and anti-adipogenics substances using three-dimensional model of adipocytes
Grantee:Patricia Santos Lopes
Support type: Regular Research Grants
FAPESP's process: 17/17844-8 - Nanostructured silica as a protective vehicle for vaccines and biomolecules
Grantee:Osvaldo Augusto Brazil Esteves Sant'Anna
Support type: Research Projects - Thematic Grants
FAPESP's process: 16/22916-5 - Development of minitablets loading papain free and complexed with cyclodextrins and sodium alginate for delivery in small intestine and colon
Grantee:Newton Andréo Filho
Support type: Regular Research Grants
FAPESP's process: 20/13204-7 - The role of mesoporous silica morphology in the incorporation of diphtheria and tetanus anatoxins: potential adjuvants for oral vaccines
Grantee:Pedro Leonidas Oseliero Filho
Support type: Scholarships abroad - Research Internship - Post-doctor
FAPESP's process: 19/08582-5 - Multi-User Equipment approved in grant 17/17844-8: thermal analyzer - TG/DTA model sta 2500 TG/DTA room temperature to 1100 °c
Grantee:Tereza da Silva Martins
Support type: Multi-user Equipment Program