Lentiviral transduction of neonatal rat ventricular myocytes preserves ultrastructural features of genetically modified cells

Autor(es)

Consonni, Silvio R. ^[1] ; de Carvalho, Anna C. P. V. ^[2] ; Mauro, Artur B. ^[2] ; Franchini, Kleber G. ^[2] ; Bajgelman, Marcio C. ^{[3, 4, 2]}

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Dept Biochem & Tissue Biol, Campinas, SP - Brazil

^[2] Ctr Res Energy & Mat CNPEM, Brazilian Natl Lab Biosci LNBio, Campinas, SP - Brazil

^[3] Univ Campinas Unicamp, Fac Pharmaceut Sci, Campinas, SP - Brazil

^[4] Univ Campinas UNICAMP, Med Sch, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: VIROLOGY; v. 562, p. 190-196, OCT 2021.
• Citações Web of Science: 0

Resumo

Preserving morphological features that are important for cell function and structure is a critical parameter for in vitro experiments with rat cardiomyocytes. Lentiviral vectors are commonly used as gene transfer tool because of its high flexibility, efficiency to deliver expression cassettes and versatility of transducing quiescent cells. The tropism of the recombinant viral particle can be determined depending on the virus envelope, which shows a specific binding to cell surface receptors on the target cell. The combination of promoter arrangement and viral envelope must be optimized to achieve a greater transduction efficiency and a higher transgene expression. In this study we explored the optimization of promoters and heterologous envelopes to transduce primary culture of neonatal rat ventricular myocytes. Our results suggest a robust expression driven by the cytomegalovirus promoter, and high efficiency transduction mediated by VSV-G envelope with no apparent compromising ultrastructural features of genetically modified cells. (AU)

• Processo FAPESP: 17/21720-2 - Papel das proteínas do disco Z na homeostase e na hipertrofia patológica de miócitos cardíacos
• Beneficiário: Sílvio Roberto Consonni
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/04458-8 - Desenvolvimento de nanopartículas biológicas para potencialização da resposta imune antitumoral
• Beneficiário: Marcio Chaim Bajgelman
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/17086-7 - Investigação da localização subcelular da quinase de adesão focal (FAK) e sua forma mutada (FAKY397F) em cardiomiócitos in vitro e em corações de camundongos transgênicos
• Beneficiário: Sílvio Roberto Consonni
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/13132-0 - Exploração de alvos moleculares para inibir células T regulatórias e potencializar resposta imune antitumoral
• Beneficiário: Marcio Chaim Bajgelman
• Modalidade de apoio: Auxílio à Pesquisa - Regular