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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Lentiviral transduction of neonatal rat ventricular myocytes preserves ultrastructural features of genetically modified cells

Full text
Author(s):
Consonni, Silvio R. [1] ; de Carvalho, Anna C. P. V. [2] ; Mauro, Artur B. [2] ; Franchini, Kleber G. [2] ; Bajgelman, Marcio C. [3, 4, 2]
Total Authors: 5
Affiliation:
[1] Univ Campinas UNICAMP, Dept Biochem & Tissue Biol, Campinas, SP - Brazil
[2] Ctr Res Energy & Mat CNPEM, Brazilian Natl Lab Biosci LNBio, Campinas, SP - Brazil
[3] Univ Campinas Unicamp, Fac Pharmaceut Sci, Campinas, SP - Brazil
[4] Univ Campinas UNICAMP, Med Sch, Campinas, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: VIROLOGY; v. 562, p. 190-196, OCT 2021.
Web of Science Citations: 0
Abstract

Preserving morphological features that are important for cell function and structure is a critical parameter for in vitro experiments with rat cardiomyocytes. Lentiviral vectors are commonly used as gene transfer tool because of its high flexibility, efficiency to deliver expression cassettes and versatility of transducing quiescent cells. The tropism of the recombinant viral particle can be determined depending on the virus envelope, which shows a specific binding to cell surface receptors on the target cell. The combination of promoter arrangement and viral envelope must be optimized to achieve a greater transduction efficiency and a higher transgene expression. In this study we explored the optimization of promoters and heterologous envelopes to transduce primary culture of neonatal rat ventricular myocytes. Our results suggest a robust expression driven by the cytomegalovirus promoter, and high efficiency transduction mediated by VSV-G envelope with no apparent compromising ultrastructural features of genetically modified cells. (AU)

FAPESP's process: 17/21720-2 - Role of Z disc proteins in homeostasis and pathological hypertrophy of cardiac myocytes
Grantee:Sílvio Roberto Consonni
Support Opportunities: Regular Research Grants
FAPESP's process: 19/04458-8 - Development of biological nanoparticles to boost antitumor immunity
Grantee:Marcio Chaim Bajgelman
Support Opportunities: Regular Research Grants
FAPESP's process: 10/17086-7 - Investigation of cellular localization focal adhesion kinase (FAK) and mutant FAK (FAKY397F) in cardiomiocytes in vitro and in genetically modified mice
Grantee:Sílvio Roberto Consonni
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 12/13132-0 - Exploration of molecular targets to inhibit regulatory T cells and potentiate antitumoral immunity
Grantee:Marcio Chaim Bajgelman
Support Opportunities: Regular Research Grants