Effects of Eugenol and Dehydrodieugenol B from Nectandra leucantha against Lipopolysaccharide (LPS)-Induced Experimental Acute Lung Inflammation

Autor(es):Mostrar menos -

Bittencourt-Mernak, I, Marcia ; Pinheiro, Nathalia M. ^{[1, 2]} ; da Silva, Rafael C. ^[3] ; Ponci, Vitor ^[3] ; Banzato, Rosana ^[2] ; Pinheiro, Aruana J. M. C. R. ^{[4, 5]} ; Olivo, Clarice R. ^[2] ; Tiberio, Iolanda F. L. C. ^[2] ; Lima Neto, Lidio G. ^{[4, 5]} ; Santana, Fernanda P. R. ^{[3, 6]} ; Lago, Joao H. G. ^[7] ; Prado, Carla M. ^{[1, 3, 2]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Biosci, BR-11015020 Santos, SP - Brazil

^[2] Univ Sao Paulo, Sch Med, Dept Med, BR-01246000 Sao Paulo, SP - Brazil

^[3] Bittencourt-Mernak, Marcia, I, Univ Fed Sao Paulo, Dept Biol Sci, BR-09913030 Diadema, SP - Brazil

^[4] Univ CEUMA, BR-65075120 Sao Luis, Maranhao - Brazil

^[5] Programa Posgrad Rede BIONORTE, BR-65055310 Sao Luis, Maranhao - Brazil

^[6] Univ Fed Sao Paulo, Dept Med Nephrol, BR-04023062 Sao Paulo, SP - Brazil

^[7] Fed Univ ABC, Ctr Nat Sci & Humanities, BR-09210170 Santo Andre, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Natural Products; v. 84, n. 8, p. 2282-2294, AUG 27 2021.
• Citações Web of Science: 0

Resumo

Acute lung injury (ALI) is an important public health problem. The present work investigated whether dehydrodieugenol B treatment, a compound isolated from Brazilian plant Nectandra leucantha (Lauraceae), modulates experimental ALI and compared the observed effects to eugenol. Effects of dehydrodieugenol B in vitro in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells were evaluated. The lung and systemic inflammatory profile, lung function, and possible mechanisms involved in BALB/C male mice (6-8 weeks) with ALI induced by LPS instillation (5 mg/kg) was assayed. Dehydrodieugenol B did not affect the cell viability and inhibited the increase in NO release and IL-1 beta and IL-6 gene expression induced by LPS. In vivo, both compounds reduced lung edema, inflammatory cells, and the IL-6 and IL-1 beta levels in bronchoalveolar lavage fluid, as well as reduced inflammatory cell infiltration and those positive to iNOS, MMP-9, and TIMP-1, and reduced the collagen content and the 8-isoprostane expression in lung tissue. Eugenol and dehydrodieugenol B also inhibited the phosphorylation of Jc-Jun-NH2 terminal Kinase (JNK), a signaling protein involved in the MAPKinase pathway. There was no effect of these compounds in lung function. Therefore, eugenol and dehydrodieugenol B ameliorates several features of experimental ALI and could be considered as a pharmacological tool to ameliorate acute lung inflammation. (AU)

• Processo FAPESP: 18/07885-1 - Biomoléculas oriundas de espécies vegetais de áreas remanescentes da Mata Atlântica e do Cerrado para tratamento de doenças tropicais negligenciadas - aspectos químicos e farmacológicos
• Beneficiário: João Henrique Ghilardi Lago
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular
• Processo FAPESP: 18/06088-0 - Efeito do tratamento com eugenol e deidrodieugenol isolados de Nectandra leucantha (Lauraceae) em modelos experimentais de doenças pulmonares agudas e crônicas.
• Beneficiário: Carla Máximo Prado
• Modalidade de apoio: Auxílio à Pesquisa - Regular