Arabidopsis-Based Dual-Layered Biological Network Analysis Elucidates Fully Modulated Pathways Related to Sugarcane Resistance on Biotrophic Pathogen Infection

Autor(es)

Rody, Hugo V. S. ^[1] ; Camargo, Luis E. A. ^[1] ; Creste, Silvana ^[2] ; Van Sluys, Marie-Anne ^[3] ; Rieseberg, Loren H. ^[4] ; Monteiro-Vitorello, Claudia B. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Genet, Escola Super Agr Luiz Queiroz, Piracicaba - Brazil

^[2] IAC Apta, Ctr Cana, Ribeirao Preto - Brazil

^[3] Univ Sao Paulo, Inst Biociencias, Dept Botan, Sao Paulo - Brazil

^[4] Univ British Columbia, Dept Bot, Vancouver, BC - Canada

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN PLANT SCIENCE; v. 12, AUG 19 2021.
• Citações Web of Science: 0

Resumo

We assembled a dual-layered biological network to study the roles of resistance gene analogs (RGAs) in the resistance of sugarcane to infection by the biotrophic fungus causing smut disease. Based on sugarcane-Arabidopsis orthology, the modeling used metabolic and protein-protein interaction (PPI) data from Arabidopsis thaliana (from Kyoto Encyclopedia of Genes and Genomes (KEGG) and BioGRID databases) and plant resistance curated knowledge for Viridiplantae obtained through text mining of the UniProt/SwissProt database. With the network, we integrated functional annotations and transcriptome data from two sugarcane genotypes that differ significantly in resistance to smut and applied a series of analyses to compare the transcriptomes and understand both signal perception and transduction in plant resistance. We show that the smut-resistant sugarcane has a larger arsenal of RGAs encompassing transcriptionally modulated subnetworks with other resistance elements, reaching hub proteins of primary metabolism. This approach may benefit molecular breeders in search of markers associated with quantitative resistance to diseases in non-model systems.</p> (AU)

• Processo FAPESP: 18/04555-0 - Padrão de expressão de genes candidatos a RGAs durante a infecção com vários patógenos
• Beneficiário: Hugo Rody Vianna Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 19/13530-4 - Modelagem, análise, e comparação de múltiplas redes metabólicas de cana-de-açúcar para investigar os mecanismos de progressão da doença do carvão
• Beneficiário: Hugo Rody Vianna Silva
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 16/17545-8 - Contribuição de genes, genomas e elementos de transposição na interação entre plantas e micro-organismos: estudo de caso em cana-de-açúcar
• Beneficiário: Marie-Anne Van Sluys
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático