Arabidopsis-Based Dual-Layered Biological Network Analysis Elucidates Fully Modulated Pathways Related to Sugarcane Resistance on Biotrophic Pathogen Infection

Author(s)

Rody, Hugo V. S. ^[1] ; Camargo, Luis E. A. ^[1] ; Creste, Silvana ^[2] ; Van Sluys, Marie-Anne ^[3] ; Rieseberg, Loren H. ^[4] ; Monteiro-Vitorello, Claudia B. ^[1]

Affiliation

^[1] Univ Sao Paulo, Dept Genet, Escola Super Agr Luiz Queiroz, Piracicaba - Brazil

^[2] IAC Apta, Ctr Cana, Ribeirao Preto - Brazil

^[3] Univ Sao Paulo, Inst Biociencias, Dept Botan, Sao Paulo - Brazil

^[4] Univ British Columbia, Dept Bot, Vancouver, BC - Canada

• Document type: Journal article
• Source: FRONTIERS IN PLANT SCIENCE; v. 12, AUG 19 2021.
• Web of Science Citations: 0

Abstract

We assembled a dual-layered biological network to study the roles of resistance gene analogs (RGAs) in the resistance of sugarcane to infection by the biotrophic fungus causing smut disease. Based on sugarcane-Arabidopsis orthology, the modeling used metabolic and protein-protein interaction (PPI) data from Arabidopsis thaliana (from Kyoto Encyclopedia of Genes and Genomes (KEGG) and BioGRID databases) and plant resistance curated knowledge for Viridiplantae obtained through text mining of the UniProt/SwissProt database. With the network, we integrated functional annotations and transcriptome data from two sugarcane genotypes that differ significantly in resistance to smut and applied a series of analyses to compare the transcriptomes and understand both signal perception and transduction in plant resistance. We show that the smut-resistant sugarcane has a larger arsenal of RGAs encompassing transcriptionally modulated subnetworks with other resistance elements, reaching hub proteins of primary metabolism. This approach may benefit molecular breeders in search of markers associated with quantitative resistance to diseases in non-model systems.</p> (AU)

• FAPESP's process: 18/04555-0 - RGAs sugarcane expression profile during infection with various pathogens
• Grantee: Hugo Rody Vianna Silva
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 19/13530-4 - Modeling, analysis, and comparison of multiple sugarcane metabolic networks to unravel mechanisms of the smut disease progression
• Grantee: Hugo Rody Vianna Silva
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• FAPESP's process: 16/17545-8 - Genes, genomes & transposable elements contribution to plant-microbe interaction: a sugarcane study case
• Grantee: Marie-Anne Van Sluys
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants