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Proteomic analysis of leaves of sugarcane cultivars and genotypes

Grant number: 11/11308-0
Support Opportunities:Scholarships in Brazil - Doctorate (Direct)
Effective date (Start): October 01, 2011
Effective date (End): September 30, 2016
Field of knowledge:Biological Sciences - Biochemistry - Molecular Biology
Principal Investigator:Solange Maria de Toledo Serrano
Grantee:Eduardo Shigueo Kitano
Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
Associated research grant:98/14307-9 - Center for Applied Toxinology, AP.CEPID


Brazil is the biggest producer of sugarcane in the world. In 2008/09, the Brazilian production was around 570 millions of tons. Moreover, the country is emerging as a worldwide leader in sugar production and maintains the second position in ethanol production. In a research consortium focused on studying the sugarcane genome (SUCEST Project), several researchers were responsible for sequencing 237,954 ESTs derived from 26 cDNA libraries, covering approximately 90% of the expressed sugarcane genome. Following the identification of ESTs, several projects based on transcriptomic analysis of this grass have been carried out ( along with the development of tools to assess the role of the components involved in signal transduction pathways. Research on sugarcane genome focused on the genes that encode proteins involved in the signal transduction pathways is important since it accelerates the identification of genes responsible for desirable qualities, allowing genetic manipulation based on molecular biology and transgenic techniques. On the other hand, it is known that mRNA levels are not always directly correlated with protein expression levels. Therefore, the study of the sugarcane proteome is important to identify proteins that are actually expressed, complementing the genome studies, and provides predictive information about proteome composition of different cultivars. The elucidation of the genomes of Arabidopsis and Oryza sativa was an important step for genomics and proteomics, allowing for the development and the establishment of techniques for proteomic studies applied to the analysis of plant proteomes, based on the predictive information derived from their genomes. It is important to emphasize that the sugarcane genome is being sequenced and the evidence of the presence of a protein can confirm gene models with observed predictors. Considering the extreme importance of the sugarcane cultivation, the interest in sucrose yield improvement and the lack of proteomic studies, the objective of this project is to analyze the proteome of leaves of two species of the genus Saccharum (S. officinarum and S. spontaneum) and a hybrid cultivar (SP80-3280), identifying differentially expressed proteins between the varieties and correlating the results with those obtained from genomic studies. In the future, the results of this study can assist in establishing the methods that will be used to define sugarcane gene networks. (AU)

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Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DIAS, GABRIELA S.; KITANO, EDUARDO S.; PAGOTTO, ANA H.; SANT'ANNA, SAVIO S.; ROCHA, MARISA M. T.; ZELANIS, ANDRE; SERRANO, SOLANGE M. T.. Individual Variability in the Venom Proteome of Juvenile Bothrops jararaca Specimens. JOURNAL OF PROTEOME RESEARCH, v. 12, n. 10, p. 4585-4598, . (11/11308-0, 98/14307-9)
DIAS, MATHEUS H.; KITANO, EDUARDO S.; ZELANIS, ANDRE; IWAI, LEO K.. Proteomics and drug discovery in cancer. DRUG DISCOVERY TODAY, v. 21, n. 2, p. 264-277, . (12/20186-9, 11/11308-0, 14/06579-3, 13/07467-1)
YAMASHIRO, EDSON T.; OLIVEIRA, ANA K.; KITANO, EDUARDO S.; MENEZES, MILENE C.; JUNQUEIRA-DE-AZEVEDO, INACIO L.; LEME, ADRIANA F. PAES; SERRANO, SOLANGE M. T.. Proteoforms of the platelet-aggregating enzyme PA-BJ, a serine proteinase from Bothrops jararaca venom. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, v. 1844, n. 12, p. 2068-2076, . (13/07467-1, 11/11308-0, 11/08514-8, 98/14307-9)
KITANO, EDUARDO S.; GARCIA, THALITA C.; MENEZES, MILENE C.; TASHIMA, ALEXANDRE K.; ZELANIS, ANDRE; SERRANO, SOLANGE M. T.. Cotiarinase is a novel prothrombin activator from the venom of Bothrops cotiara. Biochimie, v. 95, n. 8, p. 1655-1659, . (11/11308-0, 11/08514-8, 98/14307-9)
MENEZES, MILENE C.; KITANO, EDUARDO S.; BAUER, VERENA C.; OLIVEIRA, ANA K.; CARARO-LOPES, EDUARDO; NISHIYAMA, JR., MILTON Y.; ZELANIS, ANDRE; SERRANO, SOLANGE M. T.. Early response of C2C12 myotubes to a sub-cytotoxic dose of hemorrhagic metalloproteinase HF3 from Bothrops jararaca venom. JOURNAL OF PROTEOMICS, v. 198, p. 163-176, . (13/07467-1, 15/23691-4, 11/11308-0)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
KITANO, Eduardo Shigueo. Study on the leaf proteome of sugarcane cultivars and genotypes. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ) São Paulo.

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