athematical Description of the Enzymatic Activity of Proteins with Ionizable Groups Exhibiting Deviations from the Henderson-Hasselbalch Equatio

Autor(es)

Mercier Franco, Luis Fernando ^[1] ; Pessoa Filho, Pedro de Alcantara ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Chem Engn Sch, Av Albert Einstein 500, BR-13083852 Campinas, SP - Brazil

^[2] Univ Sao Paulo, Engn Sch, Dept Chem Engn, Av Prof Lineu Prestes 580, Bl 20, BR-05508000 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Applied Biochemistry and Biotechnology; v. 194, n. 3, SI OCT 2021.
• Citações Web of Science: 0

Resumo

The ionization equilibrium implied in the calculation of the specific activity is classically described through the Henderson-Hasselbalch equation. An extension for the description of anomalous ionization profiles using the Hill equation is presented in this communication. The proposed framework was applied to the description of the specific enzymatic activity curve as a function of pH of five enzymes presenting different ionization states in their active site. The developed equation improves the description of relative enzymatic curves that deviate from the bell curve predicted by the application of the Henderson-Hasselbalch equation, regardless of the ionization scheme related to the active site. (AU)

• Processo FAPESP: 12/23860-2 - Estudo sobre a relação entre a atividade biológica de proteínas e as condições de processo em operações de recuperação e purificação de bioprodutos
• Beneficiário: Pedro de Alcântara Pessôa Filho
• Modalidade de apoio: Bolsas no Exterior - Pesquisa