athematical Description of the Enzymatic Activity of Proteins with Ionizable Groups Exhibiting Deviations from the Henderson-Hasselbalch Equatio

Author(s)

Mercier Franco, Luis Fernando ^[1] ; Pessoa Filho, Pedro de Alcantara ^[2]

Affiliation

^[1] Univ Estadual Campinas, Chem Engn Sch, Av Albert Einstein 500, BR-13083852 Campinas, SP - Brazil

^[2] Univ Sao Paulo, Engn Sch, Dept Chem Engn, Av Prof Lineu Prestes 580, Bl 20, BR-05508000 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: Applied Biochemistry and Biotechnology; v. 194, n. 3, SI OCT 2021.
• Web of Science Citations: 0

Abstract

The ionization equilibrium implied in the calculation of the specific activity is classically described through the Henderson-Hasselbalch equation. An extension for the description of anomalous ionization profiles using the Hill equation is presented in this communication. The proposed framework was applied to the description of the specific enzymatic activity curve as a function of pH of five enzymes presenting different ionization states in their active site. The developed equation improves the description of relative enzymatic curves that deviate from the bell curve predicted by the application of the Henderson-Hasselbalch equation, regardless of the ionization scheme related to the active site. (AU)

• FAPESP's process: 12/23860-2 - Study of the relationship between the biological activity of proteins and process conditions in bioproduct downstream operations
• Grantee: Pedro de Alcântara Pessôa Filho
• Support Opportunities: Scholarships abroad - Research