| Texto completo | |
| Autor(es): |
Balasco Serrao, Vitor Hugo
[1, 2]
;
de Freitas Fernandes, Adriano
[1]
;
Mansor Basso, Luis Guilherme
[3, 4]
;
Scortecci, Jessica Fernandes
[1, 5]
;
Crusca Junior, Edson
[6]
;
Lopes Cornelio, Marinonio
[7]
;
Monson de Souza, Bibiana
[8]
;
Palma, Mario Sergio
[8]
;
de Oliveira Neto, Mario
[9]
;
Thiemann, Otavio Henrique
[1, 10]
Número total de Autores: 10
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Phys Inst Sao Carlos, Trabalhador Sao Carlense Av 400, BR-13566590 Sao Carlos, SP - Brazil
[2] Univ Calif Santa Cruz, Dept Chem & Biochem, 1156 High St, Santa Cruz, CA 95060 - USA
[3] Univ Sao Paulo, Fac Sci Philosophy & Letters, BR-14040901 Ribeirao Preto, SP - Brazil
[4] State Univ Northern Rio de Janeiro Darcy Ribeiro, UENF, Phys Sci Lab, Av Alberto Lamego 2000, BR-28013602 Campos Dos Goytacazes, RJ - Brazil
[5] Univ British Columbia, Dept Biochem & Mol Biol, 2350 Hlth Sci Mall, Vancouver, BC V6T 1Z3 - Canada
[6] Sao Paulo State Univ, UNESP, Chem Inst, Dept Phys Chem, BR-14800900 Araraquara, SP - Brazil
[7] Sao Paulo State Univ, UNESP, Phys Dept, Inst Biosci Letters & Exact Sci IBILCE, Sao Jose Do Rio Preto, SP - Brazil
[8] Sao Paulo State Univ, UNESP, Inst Biosci Rio Claro, Dept Gen & Appl Biol, Rio Claro, SP - Brazil
[9] Univ Estadual Paulista, Biosci Inst, BR-18618000 Botucatu, SP - Brazil
[10] Univ Fed Sao Carlos, UFSCar, Dept Genet & Evolut, BR-13565905 Sao Carlos, SP - Brazil
Número total de Afiliações: 10
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Journal of Molecular Biology; v. 433, n. 23 NOV 19 2021. |
| Citações Web of Science: | 0 |
| Resumo | |
Several molecular mechanisms are involved in the genetic code interpretation during translation, as codon degeneration for the incorporation of rare amino acids. One mechanism that stands out is selenocysteine (Sec), which requires a specific biosynthesis and incorporation pathway. In Bacteria, the Sec biosynthesis pathway has unique features compared with the eukaryote pathway as Ser to Sec conversion mechanism is accomplished by a homodecameric enzyme (selenocysteine synthase, SelA) followed by the action of an elongation factor (SelB) responsible for delivering the mature Sec-tRNA(Sec) into the ribosome by the interaction with the Selenocysteine Insertion Sequence (SECIS). Besides this mechanism being already described, the sequential events for Sec-tRNA(Sec) and SECIS specific recognition remain unclear. In this study, we determined the order of events of the interactions between the proteins and RNAs involved in Sec incorporation. Dissociation constants between SelB and the native as well as unacylated-tRNA(Sec) variants demonstrated that the acceptor stem and variable arm are essential for SelB recognition. Moreover, our data support the sequence of molecular events where GTP-activated SelB strongly interacts with SelA. tRNA(Sec). Subsequently, SelB.GTP.tRNA(Sec) recognizes the mRNA SECIS to deliver the tRNA Sec to the ribosome. SelB in complex with its specific RNAs were examined using Hydrogen/Deuterium exchange mapping that allowed the determination of the molecular envelopes and its secondary structural variations during the complex assembly. Our results demonstrate the ordering of events in Sec incorporation and contribute to the full comprehension of the tRNA(Sec) role in the Sec amino acid biosynthesis, as well as extending the knowledge of synthetic biology and the expansion of the genetic code. (C) 2021 Elsevier Ltd. All rights reserved. (AU) | |
| Processo FAPESP: | 13/17791-0 - Caracterização molecular e estrutural do Leishmania RNA vírus LRV1-4 |
| Beneficiário: | Otavio Henrique Thiemann |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 14/00206-0 - Estrutura e função dos peptídeos de fusão da glicoproteína spike do SARS-CoV |
| Beneficiário: | Luís Guilherme Mansor Basso |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 12/15777-8 - Estudos estruturais e cinéticos da enzima Desoxicitidilato desaminase (DEOC) de Schistosoma mansoni |
| Beneficiário: | Jessica Fernandes Scortecci |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |
| Processo FAPESP: | 12/23730-1 - Caracterização das interações macromoleculares das proteínas envolvidas na síntese de selenocisteínas em escherichia coli |
| Beneficiário: | Vitor Hugo Balasco Serrão |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 14/16005-4 - Caracterização das interações moleculares entre proteínas da via de síntese de selenocisteínas |
| Beneficiário: | Jessica Fernandes Scortecci |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |
| Processo FAPESP: | 98/14138-2 - Center for Structural Molecular Biotechnology |
| Beneficiário: | Glaucius Oliva |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |