Cytotoxicity and cytokine production by calcium silicate-based materials on periodontal ligament stem cells

Autor(es)

Marlus da Silva Pedrosa ^[1] ; Tomaz Alves ^[2] ; Fernando Neves Nogueira ^[3] ; Marinella Holzhausen ^[4] ; Carla Renata Sipert ^[5]

Afiliação do(s) autor(es)

^[1] University of São Paulo. School of Dentistry. Department of Biomaterials and Oral Biology - Brasil

^[2] University of São Paulo. School of Dentistry. Department of Biomaterials and Oral Biology - Brasil

^[3] University of São Paulo. School of Dentistry. Department of Biomaterials and Oral Biology - Brasil

^[4] University of São Paulo. School of Dentistry, Discipline of Periodontology. Department of Stomatology - Brasil

^[5] University of São Paulo. School of Dentistry - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Dental Journal; v. 32, n. 3, p. 65-74, 2021-11-08.

Resumo

Abstract This study investigated the effect of three commercial calcium silicate-based materials (CSBM) on cytotoxicity and pro-and anti-inflammatory cytokines production in cultured human periodontal ligament stem cells (hPDLSCs). Culture of hPDLSCs was established and characterized. Extracts of Bio-C Sealer (Angelus, Londrina, PR, Brazil), MTA Fillapex (Angelus, Londrina, PR, Brazil) and PBS Cimmo HP (Cimmo Soluções em Saúde, Pouso Alegre, MG, Brazil) were prepared by placing cement specimens (5 x 3 mm) in culture medium. Then, the extracts were serially two-fold diluted (1, 1:2, 1:4, 1:8, 1:16) and inserted into the cell-seeded wells for 24, 48 and 72 h for MTT assays. TNF-α and IL-10 cytokines were quantified by ELISA at 24h-cell supernatants. Data were analyzed by ANOVA and Tukey’s test (α = 0.05). All CSBM exhibited some cytotoxicity that varied according to extract concentration and time of evaluation. MTA Fillapex presented the highest cytotoxic effects with significant reduction of metabolic activity/cell viability when compared to Bio-C Sealer and Cimmo HP®. TNF-α was significantly upregulated by the three tested cements (p < 0.05) while only MTA Fillapex significantly upregulated IL-10 in comparison to control. Taken collectively, the results showed that PBS Cimmo HP®, Bio-C Sealer and MTA Fillapex present mild and transient cytotoxicity and slightly induced TNF-α production. MTA Fillapex upregulated IL-10 release by hPDLSCs. (AU)

• Processo FAPESP: 16/13944-5 - Papel de mediadores inflamatórios na modulação de células de papila apical humana in vitro
• Beneficiário: Carla Renata Sipert
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/23158-0 - Efeito da ativação do receptor ativado por protease do tipo 1 (par1) sobre a atividade osteogênica em membranas de células-tronco do ligamento periodontal humano
• Beneficiário: Marinella Holzhausen Caldeira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/13818-5 - Efeito da ativação do Receptor Ativado por Protease do tipo 1 (PAR1) sobre a atividade osteogênica em membranas de células-tronco do ligamento periodontal humano. ensaio in vivo
• Beneficiário: Tomaz Alves da Silva Neto
• Modalidade de apoio: Bolsas no Brasil - Mestrado