Long-term exposure of Pacu (Piaractus mesopotamicus) fish to dietary aflatoxin B-1: residues in tissues and performance

Autor(es)

Michelin, E. C. ^[1] ; Bedoya-Serna, C. M. ^[1] ; Carrion, L. C. S. ^[1] ; Godoy, S. H. S. ^[1] ; Baldin, J. C. ^[1] ; Lima, C. G. ^[2] ; Yasui, G. S. ^[3] ; Rottinghaus, G. E. ^[4] ; Sousa, R. L. M. ^[1] ; Fernandes, A. M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo FZEA USP, Fac Zootecnia & Engn Alimentos, Dept Med Vet, Av Duque de Caxias Norte 225, BR-13635900 Pirassununga, SP - Brazil

^[2] Univ Sao Paulo FZEA USP, Fac Zootecnia & Engn Alimentos, Dept Ciencias Basicas, Av Duque de Caxias Norte 225, BR-13635900 Pirassununga, SP - Brazil

^[3] Inst Chico Mendes Conservacao Biodiversidade ICMB, Rodovia SP-201, Km 6-5, BR-13630970 Pirassununga, SP - Brazil

^[4] Univ Missouri, Coll Vet Med, Vet Med Diagnost Lab, 1600 East Rollins St, Columbia, MO 65211 - USA

• Tipo de documento: Artigo Científico
• Fonte: WORLD MYCOTOXIN JOURNAL; v. 14, n. 3, SI, p. 411-419, 2021.
• Citações Web of Science: 1

Resumo

The objective of this work was to evaluate the aflatoxins residues on tissues of Pacu (Piaractus mesopotamicus) fish chronically exposed to dietary aflatoxins, also evaluating the effects on fish performance. Aflatoxins were incorporated into the extruded fish feed and the concentrations were confirmed by high performance liquid chromatography (HPLC). The experimental design comprised the following treatments: Control - feed without toxin; Treatment A: feed + 10 mu g aflatoxin B-1 (AFB(1))/kg; Treatment B: feed + 20 mu g AFB(1)/kg and Treatment C: feed + 50 mu g AFB(1)/kg. Pacu juvenile were allocated in tanks with density of one fish per litter. The experiment lasted 180 days with monthly sampling, and approximately ten fish per treatment were used to compose a sample. Biometric surveys were performed every 30 days, including standard weight and length. Aflatoxins were detected and quantified in fish muscle and liver by HPLC, using immunoafinity columns. Results showed AFB(1) deposition in the liver throughout the experiment in all treatments, but only treatment C differed significantly (P<0.05) from the others after 150 days, while in muscle there was low residual deposition. Long-term exposed fish had negative influence in weight and length. The higher mortality was observed in treatment C. Although there were AFB(1) residues at low levels in liver and only a slight amount in fish muscle, it should be noted that accumulation is possible in the Pacu species. The diets contaminated with AFB(1) negatively interfered in weight and length of fish when compared to control. Therefore, it can be concluded that long- term exposure of Pacu to dietary AFB(1) can cause losses to producers. (AU)

• Processo FAPESP: 13/23556-4 - Avaliação da transferência de aflatoxinas da ração para espécies comerciais de pescado de cultivo e ocorrência de aflatoxinas em pisciculturas do estado de São Paulo
• Beneficiário: Andrezza Maria Fernandes
• Modalidade de apoio: Auxílio à Pesquisa - Regular