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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

valuation of systemic immunity in atypical cutaneous leishmaniasis caused by Leishmania (L.) infantum chagas

Texto completo
Autor(es):
Laurenti, Marcia Dalastra [1] ; Sosa-Ochoa, Wilfredo [2, 1] ; Araujo Flores, Gabriela Venicia [1] ; Sandoval Pacheco, Carmen Maria [1] ; Tomokane, Thaise Yumie [1] ; da Silva Oliveira, Luanda Mara [3, 4] ; Zuniga, Concepcion [5] ; Silveira, Fernando Tobias [6, 7] ; Pereira Corbett, Carlos Eduardo [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Med, Dept Patol, Lab Patol Molestias Infecciosas LIM 50, Sao Paulo, SP - Brazil
[2] Univ Nacl Autonoma Honduras, Inst Invest Microbiol, Tegucigalpa - Honduras
[3] Univ Sao Paulo, Inst Med Trop Sao Paulo, Sao Paulo, SP - Brazil
[4] Univ Sao Paulo, Fac Med, Dept Dermatol, Lab Dermatol & Imunodeficiencias LIM 56, Sao Paulo, SP - Brazil
[5] Hosp Escuela Tegucigalpa, Dept Vigilancia Salud, Tegucigalpa - Honduras
[6] Minist Saude, Lab Parasitol, Inst Evandro Chagas, Belem, Para - Brazil
[7] Univ Fed, Nucleo Med Trop, Belem, Para - Brazil
Número total de Afiliações: 7
Tipo de documento: Artigo Científico
Fonte: PARASITE IMMUNOLOGY; v. 44, n. 1-2 NOV 2021.
Citações Web of Science: 0
Resumo

In some central-American countries, Leishmania (L.) infantum chagasi infection can cause non-ulcerated or atypical cutaneous leishmaniasis (NUCL) in addition to the classic clinical form, visceral leishmaniasis (VL). Little is known about the host-parasite relationship that can contribute to the determination of one or another clinical form. The present study had the objective to evaluate the humoral and cellular immunity in the sera of individuals affected by NUCL to improve the comprehension of this atypical host-parasite interaction. Based on clinical and laboratory diagnosis, serum of 80 individuals was collected to evaluate the cytokines and immunoglobulins profile of NUCL (n = 47), VL patients (n = 5), and negative controls (n = 28). Cytokines were detected using Cytokine Bead Array (CBA) Human Th1/Th2/Th17 kit according to the manufacturer's instructions; class (IgG and IgM), and subclass of (IgG1 and IgG2) immunoglobulins was evaluated by ELISA using specific antigens. The concentration of TNF-alpha, IFN-gamma, IL-2 and IL-4 cytokines in NUCL, VL and control was present below the detection threshold of CBA kit. IL-6, IL-10 and IL-17A cytokines was lower in NUCL compared to LV patients. Regarding to immunoglobulins, NUCL patients produced 4.0 times more IgG than the control, while VL patients produced 6.6 times more; and IgM level was 1.6 times higher in NUCL and 2.6 times in VL patients compared to the control. Concerning the immunoglobulins subclass, only VL patients showed positive reaction for IgG1, and IgG2 did not show positive reaction among the groups. The results showed a weak cellular and humoral systemic immune response in NUCL patients. (AU)

Processo FAPESP: 17/24834-9 - Avaliação da evolução da infecção experimental em hamster por parasitos isolados de lesão cutânea atípica e visceral humana causada por Leishmania (L.) infantum chagasi no município de Amapala, Valle, Honduras
Beneficiário:Gabriela Venicia Araujo Flores
Modalidade de apoio: Bolsas no Brasil - Doutorado
Processo FAPESP: 14/50315-0 - Leishmanioses na América Latina: uma perspectiva avançada sobre fatores imunopatogenéticos da infecção cutânea e visceral, imunomoduladores da saliva de vetores flebotomíneos e exo-antígenos imunogênicos de Leishmania (L.) infantum chagasi candidatos à vacina
Beneficiário:Marcia Dalastra Laurenti
Modalidade de apoio: Auxílio à Pesquisa - Temático
Processo FAPESP: 18/04698-6 - Leishmaniose cutânea não ulcerada ou atípica causada pela Leishmania (L.) infantum chagasi no município de Amapala, Valle, Honduras: caracterização imuno-histopatológica das lesões de pele com ênfase na participação das células apresentadoras de antígenos
Beneficiário:Carmen Maria Sandoval Pacheco
Modalidade de apoio: Bolsas no Brasil - Doutorado