valuation of systemic immunity in atypical cutaneous leishmaniasis caused by Leishmania (L.) infantum chagas

Author(s)

Laurenti, Marcia Dalastra ^[1] ; Sosa-Ochoa, Wilfredo ^{[2, 1]} ; Araujo Flores, Gabriela Venicia ^[1] ; Sandoval Pacheco, Carmen Maria ^[1] ; Tomokane, Thaise Yumie ^[1] ; da Silva Oliveira, Luanda Mara ^{[3, 4]} ; Zuniga, Concepcion ^[5] ; Silveira, Fernando Tobias ^{[6, 7]} ; Pereira Corbett, Carlos Eduardo ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Med, Dept Patol, Lab Patol Molestias Infecciosas LIM 50, Sao Paulo, SP - Brazil

^[2] Univ Nacl Autonoma Honduras, Inst Invest Microbiol, Tegucigalpa - Honduras

^[3] Univ Sao Paulo, Inst Med Trop Sao Paulo, Sao Paulo, SP - Brazil

^[4] Univ Sao Paulo, Fac Med, Dept Dermatol, Lab Dermatol & Imunodeficiencias LIM 56, Sao Paulo, SP - Brazil

^[5] Hosp Escuela Tegucigalpa, Dept Vigilancia Salud, Tegucigalpa - Honduras

^[6] Minist Saude, Lab Parasitol, Inst Evandro Chagas, Belem, Para - Brazil

^[7] Univ Fed, Nucleo Med Trop, Belem, Para - Brazil

• Document type: Journal article
• Source: PARASITE IMMUNOLOGY; v. 44, n. 1-2 NOV 2021.
• Web of Science Citations: 0

Abstract

In some central-American countries, Leishmania (L.) infantum chagasi infection can cause non-ulcerated or atypical cutaneous leishmaniasis (NUCL) in addition to the classic clinical form, visceral leishmaniasis (VL). Little is known about the host-parasite relationship that can contribute to the determination of one or another clinical form. The present study had the objective to evaluate the humoral and cellular immunity in the sera of individuals affected by NUCL to improve the comprehension of this atypical host-parasite interaction. Based on clinical and laboratory diagnosis, serum of 80 individuals was collected to evaluate the cytokines and immunoglobulins profile of NUCL (n = 47), VL patients (n = 5), and negative controls (n = 28). Cytokines were detected using Cytokine Bead Array (CBA) Human Th1/Th2/Th17 kit according to the manufacturer's instructions; class (IgG and IgM), and subclass of (IgG1 and IgG2) immunoglobulins was evaluated by ELISA using specific antigens. The concentration of TNF-alpha, IFN-gamma, IL-2 and IL-4 cytokines in NUCL, VL and control was present below the detection threshold of CBA kit. IL-6, IL-10 and IL-17A cytokines was lower in NUCL compared to LV patients. Regarding to immunoglobulins, NUCL patients produced 4.0 times more IgG than the control, while VL patients produced 6.6 times more; and IgM level was 1.6 times higher in NUCL and 2.6 times in VL patients compared to the control. Concerning the immunoglobulins subclass, only VL patients showed positive reaction for IgG1, and IgG2 did not show positive reaction among the groups. The results showed a weak cellular and humoral systemic immune response in NUCL patients. (AU)

• FAPESP's process: 17/24834-9 - Evaluation of the experimental infection in hamster by isolated parasites of atypical cutaneous lesion and human visceral caused by Leishmania (L.) infantum chagasi in the municipality of Amapala, Valle, Honduras
• Grantee: Gabriela Venicia Araujo Flores
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 14/50315-0 - Leishmaniasis in Latin America: an advanced perspective on immunopathogenetic factors of cutaneous and visceral infection, immunomodulators of the sandflies vector saliva and immunogenic exo-antigens of Leishmania (L.) infantum chagasi as vaccine candidates
• Grantee: Marcia Dalastra Laurenti
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/04698-6 - Non-ulcerated or atipycal Cutaneous Leishmaniasis caused by Leishmania (L.) infantum chagasi in the municipality of Amapala, Valle, Honduras: immunohistopathological characterization of skin lesions with emphasis in the participation of antigen presenting cells
• Grantee: Carmen Maria Sandoval Pacheco
• Support Opportunities: Scholarships in Brazil - Doctorate